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TEAD1 Antibody

TEAD1 Antibody

Item no.	PRS-14-950-50uL
Manufacturer	ProSci
Amount	50 uL
Category	Antibodies Immunohistochemistry Western Blot Immunofluorescence Primary Antibodies CHiP
Type	Antibody Primary
Format	Liquid
Applications	WB, IF, IHC, CHIP
Specific against	Human, Mouse
Host	Rabbit
ECLASS 10.1	32160702
ECLASS 11.0	32160702
UNSPSC	12352203
Alias	TEAD1, AA, REF1, TCF13, TEF-1, NTEF-1, TCF-13, TEAD-1
Available
Shipping	blue ice or RT
By Research Area	Cell Cycle, Transcription
Immunogen	Recombinant fusion protein containing a sequence corresponding to amino acids 135-215 of human TEAD1 (NP_068780.2).
Applications	WB: 1:500 - 1:2000 IHC: 1:50 - 1:200 IF: 1:50 - 1:200 CHIP: 1:20 - 1:50
Positive Control 1	HeLa
Positive Control 2	MCF-7
Positive Control 3	Mouse kidney
Positive Control 4	Mouse heart3
Predicted Molecular Weight	Observed: 48kDa
Purification	Affinity purification
Clonality	Polyclonal
Isotype	IgG
Conjugate	Unconjugated
Buffer	PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Storage Conditions	Store at -20˚ C. Avoid freeze / thaw cycles.
Disclaimer	Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Modifications	None
Ncbi Official Symbol	TEAD1
Ncbi Gene Id #	7003
User Note	Optimal dilutions for each application to be determined by the researcher.
Ncbi Official Symbol	TEAD1
Ncbi Official Full Name	TEA domain family member 1 (SV40 transcriptional enhancer factor)
Ncbi Organism	Homo sapiens
Swissprot #	P28347
Background	This gene encodes a ubiquitous transcriptional enhancer factor that is a member of the TEA/ATTS domain family. This protein directs the transactivation of a wide variety of genes and, in placental cells, also acts as a transcriptional repressor. Mutations in this gene cause Sveinsson's chorioretinal atrophy. Additional transcript variants have been described but their full-length natures have not been experimentally verified.
1st Image Caption	Western blot analysis of extracts of various cell lines, using TEAD1 antibody (14-950) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 120s.
2nd Image Caption	Immunohistochemistry of paraffin-embedded human lung cancer using TEAD1 antibody (14-950) at dilution of 1:100 (40x lens).
3rd Image Caption	Immunohistochemistry of paraffin-embedded human liver cancer using TEAD1 antibody (14-950) at dilution of 1:100 (40x lens).
4th Image Caption	Immunohistochemistry of paraffin-embedded human colon carcinoma using TEAD1 antibody (14-950) at dilution of 1:100 (40x lens).
5th Image Caption	Immunohistochemistry of paraffin-embedded human esophageal cancer using TEAD1 antibody (14-950) at dilution of 1:100 (40x lens).
6th Image Caption	Immunohistochemistry of paraffin-embedded human gastric cancer using TEAD1 antibody (14-950) at dilution of 1:100 (40x lens).
7th Image Caption	Immunohistochemistry of paraffin-embedded mouse lung using TEAD1 antibody (14-950) at dilution of 1:100 (40x lens).
8th Image Caption	Immunofluorescence analysis of MCF7 cells using TEAD1 antibody (14-950).
9th Image Caption	Chromatin immunoprecipitation analysis of extracts of HeLa cell line, using TEAD1 rabbit polyclonal antibody (14-950) and rabbit IgG. The amount of immunoprecipitated DNA was checked by quantitative PCR. Histogram was constructed by the ratios of the immunoprecipitated DNA to the input.

Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.

All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.

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Amount: 50 uL

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Delivery expected until 9/6/2024

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