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U2AF1 Antibody

U2AF1 Antibody

Item no.	PRS-14-831-50uL
Manufacturer	ProSci
Amount	50 uL
Category	Oncology Antibodies By Cell Type Hematology Leukemia Immunohistochemistry Western Blot Primary Antibodies
Type	Antibody Primary
Format	Liquid
Applications	WB, IHC
Specific against	Human, Mouse, Rat
Host	Rabbit
ECLASS 10.1	32160702
ECLASS 11.0	32160702
UNSPSC	12352203
Alias	Splicing factor U2AF 35 kDa subunit, U2 auxiliary factor 35 kDa subunit, U2 small nuclear RNA auxiliary factor 1, U2 snRNP auxiliary factor small subunit, U2AF1, U2AF35, U2AFBP
Available
Shipping	blue ice or RT
By Research Area	Apoptosis, Cell Cycle
Immunogen	A synthetic peptide corresponding to a sequence within amino acids 1-100 of human U2AF1 (NP_006749.1).
Applications	WB: 1:500 - 1:2000 IHC: 1:100 - 1:200
Positive Control 1	293T
Positive Control 2	HeLa
Positive Control 3	K-562
Positive Control 4	LO2
Positive Control 5	Mouse spleen
Positive Control 6	Mouse kidney
Predicted Molecular Weight	Observed: 38kDa
Purification	Affinity purification
Clonality	Polyclonal
Isotype	IgG
Conjugate	Unconjugated
Buffer	PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Storage Conditions	Store at -20˚ C. Avoid freeze / thaw cycles.
Disclaimer	Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Modifications	None
Ncbi Official Symbol	U2AF1
Ncbi Gene Id #	7307
User Note	Optimal dilutions for each application to be determined by the researcher.
Ncbi Official Symbol	U2AF1
Ncbi Official Full Name	Splicing factor U2AF 35 kDa subunit
Ncbi Organism	Homo sapiens
Swissprot #	Q01081
Background	This gene belongs to the splicing factor SR family of genes. U2 auxiliary factor, comprising a large and a small subunit, is a non-snRNP protein required for the binding of U2 snRNP to the pre-mRNA branch site. This gene encodes the small subunit which plays a critical role in both constitutive and enhancer-dependent RNA splicing by directly mediating interactions between the large subunit and proteins bound to the enhancers. Alternatively spliced transcript variants encoding different isoforms have been identified.
1st Image Caption	Western blot analysis of extracts of various cell lines, using U2AF1 antibody (14-831) at 1:3000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 3s.

Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.

All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.

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Amount: 50 uL

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Delivery expected until 9/6/2024

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