Item no. |
PRS-14-769-50uL |
Manufacturer |
ProSci
|
Amount |
50 uL |
Category |
|
Type |
Antibody Primary |
Format |
Liquid |
Applications |
WB |
Specific against |
Mouse |
Host |
Rabbit |
ECLASS 10.1 |
32160702 |
ECLASS 11.0 |
32160702 |
UNSPSC |
12352203 |
Alias |
CP47, CP49, CTRCT12, LIFL-L, PHAKOSIN, phakinin, 49 kDa cytoskeletal protein, beaded filament protein CP49, beaded filament structural protein 2, phakinin, bfps2, Cytoskeletal protein, 49-kD, lens fiber cell beaded filament protein CP 47, lens fiber cell |
Available |
|
Shipping |
blue ice or RT |
By Research Area |
Cell Cycle, Neuroscience |
Immunogen |
Recombinant fusion protein containing a sequence corresponding to amino acids 116-415 of human BFSP2 (NP_003562.1). |
Applications |
WB: 1:500 - 1:2000 |
Positive Control 1 |
Mouse eye |
Predicted Molecular Weight |
Observed: 46kDa |
Purification |
Affinity purification |
Clonality |
Polyclonal |
Isotype |
IgG |
Conjugate |
Unconjugated |
Buffer |
PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
Storage Conditions |
Store at -20˚ C. Avoid freeze / thaw cycles. |
Disclaimer |
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only. |
Modifications |
None |
Ncbi Official Symbol |
BFSP2 |
Ncbi Gene Id # |
8419 |
User Note |
Optimal dilutions for each application to be determined by the researcher. |
Ncbi Official Symbol |
BFSP2 |
Ncbi Official Full Name |
beaded filament structural protein 2 |
Ncbi Organism |
Homo sapiens |
Swissprot # |
Q13515 |
Background |
More than 99% of the vertebrate ocular lens is comprised of terminally differentiated lens fiber cells. Two lens-specific intermediate filament-like proteins, the protein product of this gene (phakinin), and filensin, are expressed only after fiber cell differentiation has begun. Both proteins are found in a structurally unique cytoskeletal element that is referred to as the beaded filament (BF). Mutations in this gene have been associated with juvenile-onset, progressive cataracts and Dowling-Meara epidermolysis bullosa simplex. |
1st Image Caption |
Western blot analysis of extracts of mouse eye, using BFSP2 antibody (14-769) at 1:3000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 5s. |
Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.
All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.