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RPL32 Antibody

RPL32 Antibody

Item no.	PRS-14-738-50uL
Manufacturer	ProSci
Amount	50 uL
Category	Antibodies Immunohistochemistry Western Blot Immunofluorescence Primary Antibodies
Type	Antibody Primary
Format	Liquid
Applications	WB, IF, IHC
Specific against	Human, Mouse, Rat
Host	Rabbit
ECLASS 10.1	32160702
ECLASS 11.0	32160702
UNSPSC	12352203
Alias	RPL32, L32, PP9932
Available
Shipping	blue ice or RT
By Research Area	Other
Immunogen	Recombinant fusion protein containing a sequence corresponding to amino acids 1-135 of human RPL32 (NP_000985.1).
Applications	WB: 1:500 - 1:2000 IHC: 1:50 - 1:100 IF: 1:50 - 1:100
Positive Control 1	A-549
Positive Control 2	HepG2
Positive Control 3	NIH/3T3
Positive Control 4	Mouse liver
Positive Control 5	Mouse stomach
Positive Control 6	Mouse spleen
Predicted Molecular Weight	Observed: 16kDa
Purification	Affinity purification
Clonality	Polyclonal
Isotype	IgG
Conjugate	Unconjugated
Buffer	PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Storage Conditions	Store at -20˚ C. Avoid freeze / thaw cycles.
Disclaimer	Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Modifications	None
Ncbi Official Symbol	RPL32
Ncbi Gene Id #	6161
User Note	Optimal dilutions for each application to be determined by the researcher.
Ncbi Official Symbol	RPL32
Ncbi Official Full Name	ribosomal protein L32
Ncbi Organism	Homo sapiens
Swissprot #	P62910
Background	Ribosomes, the organelles that catalyze protein synthesis, consist of a small 40S subunit and a large 60S subunit. Together these subunits are composed of 4 RNA species and approximately 80 structurally distinct proteins. This gene encodes a ribosomal protein that is a component of the 60S subunit. The protein belongs to the L32E family of ribosomal proteins. It is located in the cytoplasm. Although some studies have mapped this gene to 3q13.3-q21, it is believed to map to 3p25-p24. As is typical for genes encoding ribosomal proteins, there are multiple processed pseudogenes of this gene dispersed through the genome. Alternatively spliced transcript variants encoding the same protein have been observed for this gene.
1st Image Caption	Western blot analysis of extracts of various cell lines, using RPL32 antibody (14-738) at 1:3000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.
2nd Image Caption	Immunofluorescence analysis of L929 cells using RPL32 Polyclonal Antibody (14-738) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
3rd Image Caption	Immunofluorescence analysis of U-2 OS cells using RPL32 Polyclonal Antibody (14-738) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.

All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.

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Amount: 50 uL

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Delivery expected until 10/4/2024

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