Item no. |
PRS-14-738-50uL |
Manufacturer |
ProSci
|
Amount |
50 uL |
Category |
|
Type |
Antibody Primary |
Format |
Liquid |
Applications |
WB, IF, IHC |
Specific against |
Human, Mouse, Rat |
Host |
Rabbit |
ECLASS 10.1 |
32160702 |
ECLASS 11.0 |
32160702 |
UNSPSC |
12352203 |
Alias |
RPL32, L32, PP9932 |
Available |
|
Shipping |
blue ice or RT |
By Research Area |
Other |
Immunogen |
Recombinant fusion protein containing a sequence corresponding to amino acids 1-135 of human RPL32 (NP_000985.1). |
Applications |
WB: 1:500 - 1:2000
IHC: 1:50 - 1:100
IF: 1:50 - 1:100 |
Positive Control 1 |
A-549 |
Positive Control 2 |
HepG2 |
Positive Control 3 |
NIH/3T3 |
Positive Control 4 |
Mouse liver |
Positive Control 5 |
Mouse stomach |
Positive Control 6 |
Mouse spleen |
Predicted Molecular Weight |
Observed: 16kDa |
Purification |
Affinity purification |
Clonality |
Polyclonal |
Isotype |
IgG |
Conjugate |
Unconjugated |
Buffer |
PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
Storage Conditions |
Store at -20˚ C. Avoid freeze / thaw cycles. |
Disclaimer |
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only. |
Modifications |
None |
Ncbi Official Symbol |
RPL32 |
Ncbi Gene Id # |
6161 |
User Note |
Optimal dilutions for each application to be determined by the researcher. |
Ncbi Official Symbol |
RPL32 |
Ncbi Official Full Name |
ribosomal protein L32 |
Ncbi Organism |
Homo sapiens |
Swissprot # |
P62910 |
Background |
Ribosomes, the organelles that catalyze protein synthesis, consist of a small 40S subunit and a large 60S subunit. Together these subunits are composed of 4 RNA species and approximately 80 structurally distinct proteins. This gene encodes a ribosomal protein that is a component of the 60S subunit. The protein belongs to the L32E family of ribosomal proteins. It is located in the cytoplasm. Although some studies have mapped this gene to 3q13.3-q21, it is believed to map to 3p25-p24. As is typical for genes encoding ribosomal proteins, there are multiple processed pseudogenes of this gene dispersed through the genome. Alternatively spliced transcript variants encoding the same protein have been observed for this gene. |
1st Image Caption |
Western blot analysis of extracts of various cell lines, using RPL32 antibody (14-738) at 1:3000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s. |
2nd Image Caption |
Immunofluorescence analysis of L929 cells using RPL32 Polyclonal Antibody (14-738) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining. |
3rd Image Caption |
Immunofluorescence analysis of U-2 OS cells using RPL32 Polyclonal Antibody (14-738) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining. |
Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.
All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.