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HIBADH Antibody

HIBADH Antibody

Item no.	PRS-14-556-50uL
Manufacturer	ProSci
Amount	50 uL
Category	Antibodies Western Blot Primary Antibodies
Type	Antibody Primary
Format	Liquid
Applications	WB
Specific against	Human, Mouse, Rat
Host	Rabbit
ECLASS 10.1	32160702
ECLASS 11.0	32160702
UNSPSC	12352203
Alias	HIBADH, MGC40361, NS5ATP1
Available
Shipping	blue ice or RT
By Research Area	Cancer, Cell Cycle, Signal Transduction
Immunogen	Recombinant fusion protein containing a sequence corresponding to amino acids 37-336 of human HIBADH (NP_689953.1).
Applications	WB: 1:1000 - 1:3000
Positive Control 1	A-431
Positive Control 2	A-549
Positive Control 3	HepG2
Positive Control 4	293T
Positive Control 5	Mouse heart
Positive Control 6	Mouse liver
Predicted Molecular Weight	Observed: 35kDa
Purification	Affinity purification
Clonality	Polyclonal
Isotype	IgG
Conjugate	Unconjugated
Buffer	PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Storage Conditions	Store at -20˚ C. Avoid freeze / thaw cycles.
Disclaimer	Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Modifications	None
Ncbi Official Symbol	HIBADH
Ncbi Gene Id #	11112
User Note	Optimal dilutions for each application to be determined by the researcher.
Ncbi Official Symbol	HIBADH
Ncbi Official Full Name	3-hydroxyisobutyrate dehydrogenase
Ncbi Organism	Homo sapiens
Swissprot #	P31937
Background	This gene encodes a mitochondrial 3-hydroxyisobutyrate dehydrogenase enzyme. The encoded protein plays a critical role in the catabolism of L-valine by catalyzing the oxidation of 3-hydroxyisobutyrate to methylmalonate semialdehyde.
1st Image Caption	Western blot analysis of extracts of various cell lines, using HIBADH antibody (14-556) at 1:3000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 20s.

Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.

All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.

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Amount: 50 uL

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