HDAC3 Antibody

Apoptosis, Cancer, Cell Cycle, Immunology, Signal Transduction, Stem Cell

WB: 1:500 - 1:2000

IHC: 1:50 - 1:200

IF: 1:50 - 1:200

Observed: 48kDa

Polyclonal

Unconjugated

Store at -20˚ C. Avoid freeze / thaw cycles.

None

8841

HDAC3

Homo sapiens

Histones play a critical role in transcriptional regulation, cell cycle progression, and developmental events. Histone acetylation/deacetylation alters chromosome structure and affects transcription factor access to DNA. The protein encoded by this gene belongs to the histone deacetylase/acuc/apha family. It has histone deacetylase activity and represses transcription when tethered to a promoter. It may participate in the regulation of transcription through its binding with the zinc-finger transcription factor YY1. This protein can also down-regulate p53 function and thus modulate cell growth and apoptosis. This gene is regarded as a potential tumor suppressor gene.

Western blot analysis of extracts of mouse brain, using HDAC3 antibody (14-495) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Enhanced Kit.
Exposure time: 90s.

Immunohistochemistry of paraffin-embedded human lung cancer using HDAC3 antibody (14-495) at dilution of 1:100 (40x lens).

Immunohistochemistry of paraffin-embedded human stomach using HDAC3 antibody (14-495) at dilution of 1:100 (40x lens).