Item no. |
PRS-14-453-50uL |
Manufacturer |
ProSci
|
Amount |
50 uL |
Category |
|
Type |
Antibody Primary |
Format |
Liquid |
Applications |
WB, IF, IHC |
Specific against |
Human, Mouse, Rat |
Host |
Rabbit |
ECLASS 10.1 |
32160702 |
ECLASS 11.0 |
32160702 |
UNSPSC |
12352203 |
Alias |
26S proteasome non-ATPase regulatory subunit 13, 26S proteasome regulatory subunit RPN9, 26S proteasome regulatory subunit S11, 26S proteasome regulatory subunit p405, PSMD13 |
Available |
|
Shipping |
blue ice or RT |
By Research Area |
Cell Cycle |
Immunogen |
Recombinant fusion protein containing a sequence corresponding to amino acids 1-250 of human PSMD13 (NP_002808.3). |
Applications |
WB: 1:500 - 1:2000
IHC: 1:50 - 1:200
IF: 1:50 - 1:200 |
Positive Control 1 |
HeLa |
Positive Control 2 |
HepG2 |
Positive Control 3 |
SW620 |
Positive Control 4 |
Mouse liver |
Positive Control 5 |
Mouse testis |
Positive Control 6 |
Mouse skeletal muscle |
Predicted Molecular Weight |
Observed: 43kDa |
Purification |
Affinity purification |
Clonality |
Polyclonal |
Isotype |
IgG |
Conjugate |
Unconjugated |
Buffer |
PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
Storage Conditions |
Store at -20˚ C. Avoid freeze / thaw cycles. |
Disclaimer |
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only. |
Modifications |
None |
Ncbi Official Symbol |
PSMD13 |
Ncbi Gene Id # |
5719 |
User Note |
Optimal dilutions for each application to be determined by the researcher. |
Ncbi Official Symbol |
PSMD13 |
Ncbi Official Full Name |
26S proteasome non-ATPase regulatory subunit 13 |
Ncbi Organism |
Homo sapiens |
Swissprot # |
Q9UNM6 |
Background |
The 26S proteasome is a multicatalytic proteinase complex with a highly ordered structure composed of 2 complexes, a 20S core and a 19S regulator. The 20S core is composed of 4 rings of 28 non-identical subunits; 2 rings are composed of 7 alpha subunits and 2 rings are composed of 7 beta subunits. The 19S regulator is composed of a base, which contains 6 ATPase subunits and 2 non-ATPase subunits, and a lid, which contains up to 10 non-ATPase subunits. Proteasomes are distributed throughout eukaryotic cells at a high concentration and cleave peptides in an ATP/ubiquitin-dependent process in a non-lysosomal pathway. An essential function of a modified proteasome, the immunoproteasome, is the processing of class I MHC peptides. This gene encodes a non-ATPase subunit of the 19S regulator. Two transcripts encoding different isoforms have been described. |
1st Image Caption |
Western blot analysis of extracts of various cell lines, using PSMD13 Antibody (14-453) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 120s. |
2nd Image Caption |
Immunohistochemistry of paraffin-embedded rat brain using PSMD13 antibody (14-453) at dilution of 1:100 (40x lens). |
3rd Image Caption |
Immunohistochemistry of paraffin-embedded human esophagus using PSMD13 antibody (14-453) at dilution of 1:100 (40x lens). |
4th Image Caption |
Immunofluorescence analysis of U2OS cells using PSMD13 antibody (14-453). Blue: DAPI for nuclear staining. |
Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.
All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.