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PSMD13 Antibody

PSMD13 Antibody

Item no.	PRS-14-453-50uL
Manufacturer	ProSci
Amount	50 uL
Category	Antibodies Immunohistochemistry Western Blot Immunofluorescence Primary Antibodies
Type	Antibody Primary
Format	Liquid
Applications	WB, IF, IHC
Specific against	Human, Mouse, Rat
Host	Rabbit
ECLASS 10.1	32160702
ECLASS 11.0	32160702
UNSPSC	12352203
Alias	26S proteasome non-ATPase regulatory subunit 13, 26S proteasome regulatory subunit RPN9, 26S proteasome regulatory subunit S11, 26S proteasome regulatory subunit p405, PSMD13
Available
Shipping	blue ice or RT
By Research Area	Cell Cycle
Immunogen	Recombinant fusion protein containing a sequence corresponding to amino acids 1-250 of human PSMD13 (NP_002808.3).
Applications	WB: 1:500 - 1:2000 IHC: 1:50 - 1:200 IF: 1:50 - 1:200
Positive Control 1	HeLa
Positive Control 2	HepG2
Positive Control 3	SW620
Positive Control 4	Mouse liver
Positive Control 5	Mouse testis
Positive Control 6	Mouse skeletal muscle
Predicted Molecular Weight	Observed: 43kDa
Purification	Affinity purification
Clonality	Polyclonal
Isotype	IgG
Conjugate	Unconjugated
Buffer	PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Storage Conditions	Store at -20˚ C. Avoid freeze / thaw cycles.
Disclaimer	Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Modifications	None
Ncbi Official Symbol	PSMD13
Ncbi Gene Id #	5719
User Note	Optimal dilutions for each application to be determined by the researcher.
Ncbi Official Symbol	PSMD13
Ncbi Official Full Name	26S proteasome non-ATPase regulatory subunit 13
Ncbi Organism	Homo sapiens
Swissprot #	Q9UNM6
Background	The 26S proteasome is a multicatalytic proteinase complex with a highly ordered structure composed of 2 complexes, a 20S core and a 19S regulator. The 20S core is composed of 4 rings of 28 non-identical subunits; 2 rings are composed of 7 alpha subunits and 2 rings are composed of 7 beta subunits. The 19S regulator is composed of a base, which contains 6 ATPase subunits and 2 non-ATPase subunits, and a lid, which contains up to 10 non-ATPase subunits. Proteasomes are distributed throughout eukaryotic cells at a high concentration and cleave peptides in an ATP/ubiquitin-dependent process in a non-lysosomal pathway. An essential function of a modified proteasome, the immunoproteasome, is the processing of class I MHC peptides. This gene encodes a non-ATPase subunit of the 19S regulator. Two transcripts encoding different isoforms have been described.
1st Image Caption	Western blot analysis of extracts of various cell lines, using PSMD13 Antibody (14-453) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 120s.
2nd Image Caption	Immunohistochemistry of paraffin-embedded rat brain using PSMD13 antibody (14-453) at dilution of 1:100 (40x lens).
3rd Image Caption	Immunohistochemistry of paraffin-embedded human esophagus using PSMD13 antibody (14-453) at dilution of 1:100 (40x lens).
4th Image Caption	Immunofluorescence analysis of U2OS cells using PSMD13 antibody (14-453). Blue: DAPI for nuclear staining.

Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.

All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.

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Delivery expected until 10/4/2024

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