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Iba1 Antibody

Iba1 Antibody

Item no.	PRS-14-395-50uL
Manufacturer	ProSci
Amount	50 uL
Category	Neuroscience Neuroinflammation Antibodies Cell Biology Cell Culture Cytokines Immunohistochemistry Western Blot Immunofluorescence Primary Antibodies
Type	Antibody Primary
Format	Liquid
Applications	WB, IF, IHC
Specific against	Human, Mouse, Rat
Host	Rabbit
ECLASS 10.1	32160702
ECLASS 11.0	32160702
UNSPSC	12352203
Alias	AIF1, allograft inflammatory factor 1, IBA1, AIF-1, IRT-1, interferon gamma responsive transcript, ionized calcium-binding adapter molecule, Daintain, G1
Available
Shipping	blue ice or RT
By Research Area	Cell Cycle, Immunology, Innate Immunity, Neuroscience
Immunogen	Recombinant fusion protein containing a sequence corresponding to amino acids 1-147 of human Iba1 (NP_001614.3).
Applications	WB: 1:500 - 1:2000 IHC: 1:50 - 1:100 IF: 1:50 - 1:100
Positive Control 1	Rat spleen
Predicted Molecular Weight	Observed: 16kDa
Purification	Affinity purification
Clonality	Polyclonal
Isotype	IgG
Conjugate	Unconjugated
Buffer	PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Storage Conditions	Store at -20˚ C. Avoid freeze / thaw cycles.
Disclaimer	Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Modifications	None
Ncbi Official Symbol	AIF1
Ncbi Gene Id #	199
User Note	Optimal dilutions for each application to be determined by the researcher.
Ncbi Official Symbol	AIF1
Ncbi Official Full Name	allograft inflammatory factor 1
Ncbi Organism	Homo sapiens
Swissprot #	P55008
Background	This gene encodes a protein that binds actin and calcium. This gene is induced by cytokines and interferon and may promote macrophage activation and growth of vascular smooth muscle cells and T-lymphocytes. Polymorphisms in this gene may be associated with systemic sclerosis. Alternative splicing results in multiple transcript variants, but the full-length and coding nature of some of these variants is not certain.
1st Image Caption	Western blot analysis of extracts of rat spleen, using Iba1 antibody (14-395) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.
2nd Image Caption	Immunohistochemistry of paraffin-embedded rat spinal cord using Iba1 antibody (14-395) at dilution of 1:100 (40x lens).
3rd Image Caption	Immunohistochemistry of paraffin-embedded rat spleen using Iba1 antibody (14-395) at dilution of 1:100 (40x lens).
4th Image Caption	Immunohistochemistry of paraffin-embedded mouse spleen using Iba1 antibody (14-395) at dilution of 1:100 (40x lens).

Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.

All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.

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Delivery expected until 9/6/2024

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