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NAGS Antibody

NAGS Antibody

Item no.	PRS-14-253-50uL
Manufacturer	ProSci
Amount	50 uL
Category	Antibodies Western Blot Primary Antibodies
Type	Antibody Primary
Format	Liquid
Applications	WB
Specific against	Human, Mouse, Rat
Host	Rabbit
ECLASS 10.1	32160702
ECLASS 11.0	32160702
UNSPSC	12352203
Alias	NAGS, AGAS, ARGA, MGC133025
Available
Shipping	blue ice or RT
By Research Area	Cancer, Signal Transduction
Immunogen	Recombinant fusion protein containing a sequence corresponding to amino acids 295-534 of human NAGS (NP_694551.1).
Applications	WB: 1:500 - 1:1000
Positive Control 1	293T
Positive Control 2	K-562
Positive Control 3	Mouse liver
Positive Control 4	Mouse small intestine
Positive Control 5	Mouse testis
Positive Control 6	Rat liver
Predicted Molecular Weight	Observed: 58kDa
Purification	Affinity purification
Clonality	Polyclonal
Isotype	IgG
Conjugate	Unconjugated
Buffer	PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Storage Conditions	Store at -20˚ C. Avoid freeze / thaw cycles.
Disclaimer	Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Modifications	None
Ncbi Official Symbol	NAGS
Ncbi Gene Id #	162417
User Note	Optimal dilutions for each application to be determined by the researcher.
Ncbi Official Symbol	NAGS
Ncbi Official Full Name	N-acetylglutamate synthase
Ncbi Organism	Homo sapiens
Swissprot #	Q8N159
Background	The N-acetylglutamate synthase gene encodes a mitochondrial enzyme that catalyzes the formation of N-acetylglutamate (NAG) from glutamate and acetyl coenzyme-A. NAG is a cofactor of carbamyl phosphate synthetase I (CPSI), the first enzyme of the urea cycle in mammals. This gene may regulate ureagenesis by altering NAG availability and, thereby, CPSI activity. Deficiencies in N-acetylglutamate synthase have been associated with hyperammonemia.
1st Image Caption	Western blot analysis of extracts of various cell lines, using NAGS antibody (14-253) at 1:3000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.

Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.

All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.

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Amount: 50 uL

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Delivery expected until 10/4/2024

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