Item no. |
PRS-14-201-50uL |
Manufacturer |
ProSci
|
Amount |
50 uL |
Category |
|
Type |
Antibody Primary |
Format |
Liquid |
Applications |
WB, IHC |
Specific against |
Human, Mouse, Rat |
Host |
Rabbit |
ECLASS 10.1 |
32160702 |
ECLASS 11.0 |
32160702 |
UNSPSC |
12352203 |
Alias |
beta4Gal-T3, beta-1,4-galactosyltransferase 3, N-acetyllactosamine synthase, UDP-Gal:beta-GlcNAc beta-1,4-galactosyltransferase 3, UDP-Gal:betaGlcNAc beta 1,4- galactosyltransferase, polypeptide 3, UDP-galactose:beta-N-acetylglucosamine beta-1,4-galactosy |
Available |
|
Shipping |
blue ice or RT |
By Research Area |
Other |
Immunogen |
A synthetic peptide of human B4GALT3 |
Applications |
WB: 1:500 - 1:2000
IHC: 1:50 - 1:100 |
Positive Control 1 |
HeLa |
Predicted Molecular Weight |
Observed: 44kDa |
Purification |
Affinity purification |
Clonality |
Polyclonal |
Isotype |
IgG |
Conjugate |
Unconjugated |
Buffer |
PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
Storage Conditions |
Store at -20˚ C. Avoid freeze / thaw cycles. |
Disclaimer |
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only. |
Modifications |
None |
Ncbi Official Symbol |
B4GALT3 |
Ncbi Gene Id # |
8703 |
User Note |
Optimal dilutions for each application to be determined by the researcher. |
Ncbi Official Symbol |
B4GALT3 |
Ncbi Official Full Name |
beta-1, 4-galactosyltransferase 3 |
Ncbi Organism |
Homo sapiens |
Swissprot # |
O60512 |
Background |
This gene is one of seven beta-1, 4-galactosyltransferase (beta4GalT) genes. They encode type II membrane-bound glycoproteins that appear to have exclusive specificity for the donor substrate UDP-galactose; all transfer galactose in a beta1, 4 linkage to similar acceptor sugars: GlcNAc, Glc, and Xyl. Each beta4GalT has a distinct function in the biosynthesis of different glycoconjugates and saccharide structures. As type II membrane proteins, they have an N-terminal hydrophobic signal sequence that directs the protein to the Golgi apparatus and which then remains uncleaved to function as a transmembrane anchor. By sequence similarity, the beta4GalTs form four groups: beta4GalT1 and beta4GalT2, beta4GalT3 and beta4GalT4, beta4GalT5 and beta4GalT6, and beta4GalT7. This gene encodes an enzyme that may be mainly involved in the synthesis of the first N-acetyllactosamine unit of poly-N-acetyllactosamine chains. Multiple alternatively spliced transcript variants encoding the same protein have been found for this gene. |
1st Image Caption |
Western blot analysis of extracts of HeLa cells, using B4GALT3 antibody (14-201). Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. |
Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.
All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.