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FXN / Frataxin Antibody

FXN / Frataxin Antibody

Item no.	PRS-14-142-50uL
Manufacturer	ProSci
Amount	50 uL
Category	Neuroscience Aging & Neurological Disorders Neural Signaling Antibodies Immunohistochemistry Western Blot Immunofluorescence Primary Antibodies
Type	Antibody Primary
Format	Liquid
Applications	WB, IF, IHC
Specific against	Human, Mouse, Rat
Host	Rabbit
ECLASS 10.1	32160702
ECLASS 11.0	32160702
UNSPSC	12352203
Alias	Frataxin, mitochondrial, Friedreich ataxia protein, Fxn, Frataxin intermediate form, i-FXN, Frataxin(56-210), m56-FXN, Frataxin(78-210), d-FXN, m78-FXN, Frataxin mature form, Frataxin(81-210), m81-FXN, FXN, FRDA, X25
Available
Shipping	blue ice or RT
By Research Area	Signal Transduction
Immunogen	Recombinant fusion protein containing a sequence corresponding to amino acids 42-210 of human FXN / Frataxin (NP_000135.2).
Applications	WB: 1:500 - 1:2000 IHC: 1:50 - 1:200 IF: 1:50 - 1:200
Positive Control 1	K-562
Positive Control 2	Jurkat
Positive Control 3	Raji
Positive Control 4	DU145
Positive Control 5	Mouse kidney
Positive Control 6	Mouse liver
Predicted Molecular Weight	Observed: 13kDa
Purification	Affinity purification
Clonality	Polyclonal
Isotype	IgG
Conjugate	Unconjugated
Buffer	PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Storage Conditions	Store at -20˚ C. Avoid freeze / thaw cycles.
Disclaimer	Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Modifications	None
Ncbi Official Symbol	FXN
Ncbi Gene Id #	2395
User Note	Optimal dilutions for each application to be determined by the researcher.
Ncbi Official Symbol	FXN
Ncbi Official Full Name	Frataxin, mitochondrial
Ncbi Organism	Homo sapiens
Swissprot #	Q16595
Background	This nuclear gene encodes a mitochondrial protein which belongs to the FRATAXIN family. The protein functions in regulating mitochondrial iron transport and respiration. The expansion of intronic trinucleotide repeat GAA from 8-33 repeats to >90 repeats results in Friedreich ataxia. Alternative splicing results in multiple transcript variants.
1st Image Caption	Western blot analysis of extracts of various cell lines, using FXN / Frataxin antibody (14-142) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.
2nd Image Caption	Immunofluorescence analysis of 293T cells using FXN / Frataxin antibody (14-142) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
3rd Image Caption	Immunofluorescence analysis of HeLa cells using FXN / Frataxin antibody (14-142) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.

All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.

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Amount: 50 uL

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Delivery expected until 9/6/2024

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