Item no. |
PRS-14-002-50uL |
Manufacturer |
ProSci
|
Amount |
50 uL |
Category |
|
Type |
Antibody Primary |
Format |
Liquid |
Applications |
WB, IF |
Specific against |
Human, Mouse, Rat |
Host |
Rabbit |
ECLASS 10.1 |
32160702 |
ECLASS 11.0 |
32160702 |
UNSPSC |
12352203 |
Alias |
IL1B, interleukin 1, beta, IL-1, IL1-BETA, IL1F2, IL-1 beta, catabolin, interleukin-1 beta, preinterleukin 1 beta, pro-interleukin-1-beta |
Available |
|
Shipping |
blue ice or RT |
By Research Area |
Apoptosis, Cell Cycle, Chemokines & Cytokines, Growth Factors, Immunology, Innate Immunity, Neuroscience, Obesity |
Immunogen |
Recombinant fusion protein containing a sequence corresponding to amino acids 124-223 of human IL1B (NP_000567.1). |
Applications |
WB: 1:500 - 1:2000
IF: 1:50 - 1:200 |
Positive Control 1 |
THP-1 |
Predicted Molecular Weight |
Observed: 37kDa |
Purification |
Affinity purification |
Clonality |
Polyclonal |
Isotype |
IgG |
Conjugate |
Unconjugated |
Buffer |
PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
Storage Conditions |
Store at -20˚ C. Avoid freeze / thaw cycles. |
Disclaimer |
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only. |
Modifications |
None |
Ncbi Official Symbol |
IL1B |
Ncbi Gene Id # |
3553 |
User Note |
Optimal dilutions for each application to be determined by the researcher. |
Ncbi Official Symbol |
IL1B |
Ncbi Official Full Name |
interleukin 1, beta |
Ncbi Organism |
Homo sapiens |
Swissprot # |
P01584 |
Background |
The protein encoded by this gene is a member of the interleukin 1 cytokine family. This cytokine is produced by activated macrophages as a proprotein, which is proteolytically processed to its active form by caspase 1 (CASP1/ICE). This cytokine is an important mediator of the inflammatory response, and is involved in a variety of cellular activities, including cell proliferation, differentiation, and apoptosis. The induction of cyclooxygenase-2 (PTGS2/COX2) by this cytokine in the central nervous system (CNS) is found to contribute to inflammatory pain hypersensitivity. This gene and eight other interleukin 1 family genes form a cytokine gene cluster on chromosome 2. |
1st Image Caption |
Western blot analysis of extracts of various cell lines, using IL1B antibody (14-002) at 1:1000 dilution. THP-1 cells were treated by PMA (80 nM) for overnight. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 5s. |
2nd Image Caption |
Immunofluorescence analysis of HeLa cells using IL1B antibody (14-002) at dilution of 1:100. Blue: DAPI for nuclear staining. |
3rd Image Caption |
Immunofluorescence analysis of THP-1 cells using IL1B antibody (14-002) at dilution of 1:100. Blue: DAPI for nuclear staining. |
Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.
All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.