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PHB Antibody [A9D4]

PHB Antibody [A9D4]

Item no.	PRS-13-904-50uL
Manufacturer	ProSci
Amount	50 uL
Category	Metabolism Diabetes Glucose Homeostasis Antibodies Obesity Immunohistochemistry Immunoprecipitation Western Blot Immunofluorescence Primary Antibodies
Type	Antibody Primary
Format	Liquid
Applications	WB, IF, IP, IHC
Specific against	Human, Mouse, Rat
Host	Rabbit
ECLASS 10.1	32160702
ECLASS 11.0	32160702
UNSPSC	12352203
Alias	PHB, PHB1, HEL-215, HEL-S-54e
Available
Shipping	blue ice or RT
By Research Area	Apoptosis, Cell Cycle
Immunogen	Recombinant protein of human PHB
Applications	WB: 1:500 - 1:2000 IHC: 1:50 - 1:200 IF: 1:50 - 1:200 IP: 1:20 - 1:50
Positive Control 1	293T
Positive Control 2	Jurkat
Positive Control 3	Mouse kidney
Positive Control 4	HepG2
Predicted Molecular Weight	Observed: 30kDa
Purification	Affinity purification
Clonality	Monoclonal
Clone	[A9D4]
Isotype	IgG
Conjugate	Unconjugated
Buffer	PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Storage Conditions	Store at -20˚ C. Avoid freeze / thaw cycles.
Disclaimer	Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Modifications	None
Ncbi Official Symbol	PHB
Ncbi Gene Id #	5245
User Note	Optimal dilutions for each application to be determined by the researcher.
Ncbi Official Symbol	PHB
Ncbi Official Full Name	prohibitin
Ncbi Organism	Homo sapiens
Swissprot #	P35232
Background	This gene is evolutionarily conserved, and its product is proposed to play a role in human cellular senescence and tumor suppression. Antiproliferative activity is reported to be localized to the 3' UTR, which is proposed to function as a trans-acting regulatory RNA. Several pseudogenes of this gene have been identified. Alternative splicing results in multiple transcript variants.
1st Image Caption	Western blot analysis of extracts of various cell lines, using PHB antibody (13-904). Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST.
2nd Image Caption	Immunohistochemistry of paraffin-embedded mouse stomach using PHB antibody (13-904) at dilution of 1:100 (40x lens).
3rd Image Caption	Immunohistochemistry of paraffin-embedded human liver cancer using PHB antibody (13-904) at dilution of 1:100 (40x lens).
4th Image Caption	Immunohistochemistry of paraffin-embedded human kidney using PHB antibody (13-904) at dilution of 1:100 (40x lens).
5th Image Caption	Immunohistochemistry of paraffin-embedded mouse heart using PHB antibody (13-904) at dilution of 1:100 (40x lens).
6th Image Caption	Immunohistochemistry of paraffin-embedded mouse liver using PHB antibody (13-904) at dilution of 1:100 (40x lens).
7th Image Caption	Immunohistochemistry of paraffin-embedded mouse kidney using PHB antibody (13-904) at dilution of 1:100 (40x lens).
8th Image Caption	Immunofluorescence analysis of NIH-3T3 cells using PHB antibody (13-904).
9th Image Caption	Immunofluorescence analysis of HepG2 cells using PHB antibody (13-904).

Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.

All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.

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Amount: 50 uL

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