Item no. |
PRS-13-728-50uL |
Manufacturer |
ProSci
|
Amount |
50 uL |
Category |
|
Type |
Antibody Primary |
Format |
Liquid |
Applications |
WB, IF |
Specific against |
Human, Mouse, Rat |
Host |
Rabbit |
ECLASS 10.1 |
32160702 |
ECLASS 11.0 |
32160702 |
UNSPSC |
12352203 |
Alias |
Legumain, Asparaginyl endopeptidase, Protease, cysteine 1, LGMN, PRSC1 |
Available |
|
Shipping |
blue ice or RT |
By Research Area |
Cancer, Cell Cycle, Immunology, Signal Transduction |
Immunogen |
Recombinant fusion protein containing a sequence corresponding to amino acids 18-323 of human LGMN (NP_001008530.1). |
Applications |
WB: 1:1000 - 1:2000
IF: 1:50 - 1:200 |
Positive Control 1 |
Mouse lung |
Positive Control 2 |
Mouse liver |
Predicted Molecular Weight |
Observed: 54kDa |
Purification |
Affinity purification |
Clonality |
Polyclonal |
Isotype |
IgG |
Conjugate |
Unconjugated |
Buffer |
PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
Storage Conditions |
Store at -20˚ C. Avoid freeze / thaw cycles. |
Disclaimer |
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only. |
Modifications |
None |
Ncbi Official Symbol |
LGMN |
Ncbi Gene Id # |
5641 |
User Note |
Optimal dilutions for each application to be determined by the researcher. |
Ncbi Official Symbol |
LGMN |
Ncbi Official Full Name |
Legumain |
Ncbi Organism |
Homo sapiens |
Swissprot # |
Q99538 |
Background |
This gene encodes a cysteine protease that has a strict specificity for hydrolysis of asparaginyl bonds. This enzyme may be involved in the processing of bacterial peptides and endogenous proteins for MHC class II presentation in the lysosomal/endosomal systems. Enzyme activation is triggered by acidic pH and appears to be autocatalytic. Protein expression occurs after monocytes differentiate into dendritic cells. A fully mature, active enzyme is produced following lipopolysaccharide expression in mature dendritic cells. Overexpression of this gene may be associated with the majority of solid tumor types. This gene has a pseudogene on chromosome 13. Several alternatively spliced transcript variants have been described, but the biological validity of only two has been determined. These two variants encode the same isoform. |
1st Image Caption |
Western blot analysis of extracts of various cell lines, using LGMN antibody (13-728) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s. |
2nd Image Caption |
Immunofluorescence analysis of C6 cells using LGMN Polyclonal Antibody (13-728) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining. |
3rd Image Caption |
Immunofluorescence analysis of HeLa cells using LGMN Polyclonal Antibody (13-728) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining. |
4th Image Caption |
Immunofluorescence analysis of L929 cells using LGMN Polyclonal Antibody (13-728) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining. |
Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.
All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.