Item no. |
PRS-13-709-50uL |
Manufacturer |
ProSci
|
Amount |
50 uL |
Category |
|
Type |
Antibody Primary |
Format |
Liquid |
Applications |
WB, IP |
Specific against |
Human, Mouse, Rat |
Host |
Rabbit |
ECLASS 10.1 |
32160702 |
ECLASS 11.0 |
32160702 |
UNSPSC |
12352203 |
Alias |
TST, MGC19578, RDS |
Available |
|
Shipping |
blue ice or RT |
By Research Area |
Cancer, Signal Transduction |
Immunogen |
Recombinant fusion protein containing a sequence corresponding to amino acids 1-297 of human TST (NP_003303.2). |
Applications |
WB: 1:1000 - 1:2000
IP: 1:50 - 1:100 |
Positive Control 1 |
HeLa |
Positive Control 2 |
DU145 |
Positive Control 3 |
MCF-7 |
Positive Control 4 |
293T |
Positive Control 5 |
Mouse liver |
Positive Control 6 |
Mouse kidney |
Predicted Molecular Weight |
Observed: 36kDa |
Purification |
Affinity purification |
Clonality |
Polyclonal |
Isotype |
IgG |
Conjugate |
Unconjugated |
Buffer |
PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
Storage Conditions |
Store at -20˚ C. Avoid freeze / thaw cycles. |
Disclaimer |
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only. |
Modifications |
None |
Ncbi Official Symbol |
TST |
Ncbi Gene Id # |
7263 |
User Note |
Optimal dilutions for each application to be determined by the researcher. |
Ncbi Official Symbol |
TST |
Ncbi Official Full Name |
thiosulfate sulfurtransferase (rhodanese) |
Ncbi Organism |
Homo sapiens |
Swissprot # |
Q16762 |
Background |
This is one of two neighboring genes encoding similar proteins that each contain two rhodanese domains. The encoded protein is localized to the mitochondria and catalyzes the conversion of thiosulfate and cyanide to thiocyanate and sulfite. In addition, the protein interacts with 5S ribosomal RNA and facilitates its import into the mitochondria. Alternative splicing results in multiple transcript variants. |
1st Image Caption |
Western blot analysis of extracts of various cell lines, using TST antibody (13-709) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 5s. |
2nd Image Caption |
Immunoprecipitation analysis of 200ug extracts of A-549 cells, using 3 ug TST antibody (13-709). Western blot was performed from the immunoprecipitate using TST antibody (13-709) at a dilition of 1:1000. |
Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.
All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.