Item no. |
PRS-13-615-50uL |
Manufacturer |
ProSci
|
Amount |
50 uL |
Category |
|
Type |
Antibody Primary |
Format |
Liquid |
Applications |
WB, IHC |
Specific against |
Human, Mouse, Rat |
Host |
Rabbit |
ECLASS 10.1 |
32160702 |
ECLASS 11.0 |
32160702 |
UNSPSC |
12352203 |
Alias |
F5F8D, F5F8D2, LMAN1IP, SDNSF, multiple coagulation factor deficiency protein 2, neural stem cell-derived neuronal survival protein |
Available |
|
Shipping |
blue ice or RT |
By Research Area |
Signal Transduction |
Immunogen |
Recombinant fusion protein containing a sequence corresponding to amino acids 27-146 of human MCFD2 (NP_644808.1). |
Applications |
WB: 1:500 - 1:2000
IHC: 1:100 - 1:200 |
Positive Control 1 |
A375 |
Positive Control 2 |
SW480 |
Positive Control 3 |
Mouse liver |
Positive Control 4 |
Mouse kidney |
Positive Control 5 |
Rat kidney |
Predicted Molecular Weight |
Observed: 16kDa |
Purification |
Affinity purification |
Clonality |
Polyclonal |
Isotype |
IgG |
Conjugate |
Unconjugated |
Buffer |
PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
Storage Conditions |
Store at -20˚ C. Avoid freeze / thaw cycles. |
Disclaimer |
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only. |
Modifications |
None |
Ncbi Official Symbol |
MCFD2 |
Ncbi Gene Id # |
90411 |
User Note |
Optimal dilutions for each application to be determined by the researcher. |
Ncbi Official Symbol |
MCFD2 |
Ncbi Official Full Name |
multiple coagulation factor deficiency 2 |
Ncbi Organism |
Homo sapiens |
Swissprot # |
Q8NI22 |
Background |
This gene encodes a soluble luminal protein with two calmodulin-like EF-hand motifs at its C-terminus. This protein forms a complex with LMAN1 (lectin mannose binding protein 1; also known as ERGIC-53) that facilitates the transport of coagulation factors V (FV) and VIII (FVIII) from the endoplasmic reticulum to the Golgi apparatus via an endoplasmic reticulum Golgi intermediate compartment (ERGIC). Mutations in this gene cause combined deficiency of FV and FVIII (F5F8D); a rare autosomal recessive bleeding disorder characterized by mild to moderate bleeding and coordinate reduction in plasma FV and FVIII levels. This protein has also been shown to maintain stem cell potential in adult central nervous system and is a marker for testicular germ cell tumors. The 3' UTR of this gene contains a transposon-like human repeat element named 'THE 1'. A processed RNA pseudogene of this gene is on chromosome 6p22.1. Alternative splicing results in multiple transcript variants encoding distinct isoforms. |
1st Image Caption |
Western blot analysis of extracts of various cell lines, using MCFD2 antibody (13-615) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s. |
Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.
All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.