Item no. |
PRS-13-590-50uL |
Manufacturer |
ProSci
|
Amount |
50 uL |
Category |
|
Type |
Antibody Primary |
Format |
Liquid |
Applications |
WB, IF, IHC |
Specific against |
Human, Mouse |
Host |
Rabbit |
ECLASS 10.1 |
32160702 |
ECLASS 11.0 |
32160702 |
UNSPSC |
12352203 |
Alias |
HHR23B, HR23B, P58, UV excision repair protein RAD23 homolog B, RAD23, yeast homolog of, B, XP-C repair complementing complex 58 kDa, XP-C repair complementing protein, XP-C repair-complementing complex 58 kDa protein |
Available |
|
Shipping |
blue ice or RT |
By Research Area |
Other |
Immunogen |
A synthetic peptide corresponding to a sequence within amino acids 50-150 of human RAD23B (NP_002865.1). |
Applications |
WB: 1:500 - 1:2000
IHC: 1:100 - 1:200
IF: 1:50 - 1:200 |
Positive Control 1 |
HeLa |
Positive Control 2 |
Jurkat |
Positive Control 3 |
SKOV3 |
Positive Control 4 |
Mouse testis |
Positive Control 5 |
Mouse brain |
Positive Control 6 |
Mouse liver |
Predicted Molecular Weight |
Observed: 80kDa |
Purification |
Affinity purification |
Clonality |
Polyclonal |
Isotype |
IgG |
Conjugate |
Unconjugated |
Buffer |
PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
Storage Conditions |
Store at -20˚ C. Avoid freeze / thaw cycles. |
Disclaimer |
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only. |
Modifications |
None |
Ncbi Official Symbol |
RAD23B |
Ncbi Gene Id # |
5887 |
User Note |
Optimal dilutions for each application to be determined by the researcher. |
Ncbi Official Symbol |
RAD23B |
Ncbi Official Full Name |
RAD23 homolog B, nucleotide excision repair protein |
Ncbi Organism |
Homo sapiens |
Swissprot # |
P54727 |
Background |
The protein encoded by this gene is one of two human homologs of Saccharomyces cerevisiae Rad23, a protein involved in the nucleotide excision repair (NER). This protein was found to be a component of the protein complex that specifically complements the NER defect of xeroderma pigmentosum group C (XP-c) cell extracts in vitro. This protein was also shown to interact with, and elevate the nucleotide excision activity of 3-methyladenine-DNA glycosylase (MPG), which suggested a role in DNA damage recognition in base excision repair. This protein contains an N-terminal ubiquitin-like domain, which was reported to interact with 26S proteasome, and thus this protein may be involved in the ubiquitin mediated proteolytic pathway in cells. Alternative splicing results in multiple transcript variants encoding distinct isoforms. |
1st Image Caption |
Western blot analysis of extracts of various cell lines, using RAD23B antibody (13-590) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s. |
2nd Image Caption |
Immunohistochemistry of paraffin-embedded human prostate using RAD23B antibody (13-590) at dilution of 1:100 (40x lens). |
3rd Image Caption |
Immunohistochemistry of paraffin-embedded human liver damage using RAD23B antibody (13-590) at dilution of 1:100 (40x lens). |
4th Image Caption |
Immunohistochemistry of paraffin-embedded human stomach using RAD23B antibody (13-590) at dilution of 1:100 (40x lens). |
5th Image Caption |
Immunofluorescence analysis of U2OS cells using RAD23B antibody (13-590) at dilution of 1:100. Blue: DAPI for nuclear staining. |
Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.
All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.