Comparison

PPP1R14A Antibody

Item no. PRS-13-473-50uL
Manufacturer ProSci
Amount 50 uL
Category
Type Antibody Primary
Format Liquid
Applications WB
Specific against Human, Mouse, Rat
Host Rabbit
ECLASS 10.1 32160702
ECLASS 11.0 32160702
UNSPSC 12352203
Alias CPI-17, CPI17, PPP1INL, protein phosphatase 1 regulatory subunit 14A, 17 kDa PKC-potentiated inhibitory protein of PP1, 17-KDa protein, PKC-potentiated inhibitory protein of PP1, protein kinase C-potentiated inhibitor protein of 17 kDa
Available
Shipping
blue ice or RT
By Research Area
Signal Transduction
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 1-147 of human PPP1R14A (NP_150281.1).
Applications
WB: 1:500 - 1:2000
Positive Control 1
MCF7
Positive Control 2
Mouse spleen
Positive Control 3
Rat lung
Predicted Molecular Weight
Observed: 17kDa
Purification
Affinity purification
Clonality
Polyclonal
Isotype
IgG
Conjugate
Unconjugated
Buffer
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Storage Conditions
Store at -20˚ C. Avoid freeze / thaw cycles.
Disclaimer
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Modifications
None
Ncbi Official Symbol
PPP1R14A
Ncbi Gene Id #
94274
User Note
Optimal dilutions for each application to be determined by the researcher.
Ncbi Official Symbol
PPP1R14A
Ncbi Official Full Name
protein phosphatase 1 regulatory inhibitor subunit 14A
Ncbi Organism
Homo sapiens
Swissprot #
Q96A00
Background
The protein encoded by this gene belongs to the protein phosphatase 1 (PP1) inhibitor family. This protein is an inhibitor of smooth muscle myosin phosphatase, and has higher inhibitory activity when phosphorylated. Inhibition of myosin phosphatase leads to increased myosin phosphorylation and enhanced smooth muscle contraction. Alternatively spliced transcript variants encoding different isoforms have been noted for this gene.
1st Image Caption
Western blot analysis of extracts of various cell lines, using PPP1R14A antibody (13-473) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Enhanced Kit.
Exposure time: 60s.

Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.

All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.

Amount: 50 uL
Available: In stock
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