Item no. |
PRS-13-362-50uL |
Manufacturer |
ProSci
|
Amount |
50 uL |
Category |
|
Type |
Antibody Primary |
Format |
Liquid |
Applications |
WB, IHC |
Specific against |
Human, Mouse, Rat |
Host |
Rabbit |
ECLASS 10.1 |
32160702 |
ECLASS 11.0 |
32160702 |
UNSPSC |
12352203 |
Alias |
ID2, GIG8, ID2A, ID2H, MGC26389, bHLHb26 |
Available |
|
Shipping |
blue ice or RT |
By Research Area |
Stem Cell, Transcription |
Immunogen |
Recombinant fusion protein containing a sequence corresponding to amino acids 75-134 of human ID2 (NP_002157.2). |
Applications |
WB: 1:500 - 1:2000
IHC: 1:50 - 1:200 |
Positive Control 1 |
293T |
Predicted Molecular Weight |
Observed: 15kDa |
Purification |
Affinity purification |
Clonality |
Polyclonal |
Isotype |
IgG |
Conjugate |
Unconjugated |
Buffer |
PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
Storage Conditions |
Store at -20˚ C. Avoid freeze / thaw cycles. |
Disclaimer |
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only. |
Modifications |
None |
Ncbi Official Symbol |
ID2 |
Ncbi Gene Id # |
3398 |
User Note |
Optimal dilutions for each application to be determined by the researcher. |
Ncbi Official Symbol |
ID2 |
Ncbi Official Full Name |
inhibitor of DNA binding 2, dominant negative helix-loop-helix protein |
Ncbi Organism |
Homo sapiens |
Swissprot # |
Q02363 |
Background |
The protein encoded by this gene belongs to the inhibitor of DNA binding family, members of which are transcriptional regulators that contain a helix-loop-helix (HLH) domain but not a basic domain. Members of the inhibitor of DNA binding family inhibit the functions of basic helix-loop-helix transcription factors in a dominant-negative manner by suppressing their heterodimerization partners through the HLH domains. This protein may play a role in negatively regulating cell differentiation. A pseudogene of this gene is located on chromosome 3. |
1st Image Caption |
Western blot analysis of extracts of mouse lung, using ID2 antibody (13-362) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 90s. |
2nd Image Caption |
Immunohistochemistry of paraffin-embedded rat ovary using ID2 antibody (13-362) at dilution of 1:100 (40x lens). |
3rd Image Caption |
Immunohistochemistry of paraffin-embedded human oophoroma using ID2 antibody (13-362) at dilution of 1:100 (40x lens). |
4th Image Caption |
Immunohistochemistry of paraffin-embedded mouse liver using ID2 antibody (13-362) at dilution of 1:100 (40x lens). |
Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.
All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.