Item no. |
PRS-13-208-50uL |
Manufacturer |
ProSci
|
Amount |
50 uL |
Category |
|
Type |
Antibody Primary |
Format |
Liquid |
Applications |
WB, IF, IP |
Specific against |
Human, Mouse, Rat |
Host |
Rabbit |
ECLASS 10.1 |
32160702 |
ECLASS 11.0 |
32160702 |
UNSPSC |
12352203 |
Alias |
PEBP1, HCNP, PBP, PEBP, RKIP, HCNPpp, PEBP-1, HEL-210, HEL-S-34 |
Available |
|
Shipping |
blue ice or RT |
By Research Area |
Cancer, Cell Cycle, Neuroscience |
Immunogen |
Recombinant fusion protein containing a sequence corresponding to amino acids 1-187 of human PEBP1 (NP_002558.1). |
Applications |
WB: 1:500 - 1:2000
IF: 1:50 - 1:200
IP: 1:20 - 1:50 |
Positive Control 1 |
BT-474 |
Positive Control 2 |
293T |
Positive Control 3 |
22Rv1 |
Positive Control 4 |
A-549 |
Positive Control 5 |
PC-3 |
Positive Control 6 |
MCF7 |
Predicted Molecular Weight |
Observed: 22kDa |
Purification |
Affinity purification |
Clonality |
Polyclonal |
Isotype |
IgG |
Conjugate |
Unconjugated |
Buffer |
PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
Storage Conditions |
Store at -20˚ C. Avoid freeze / thaw cycles. |
Disclaimer |
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only. |
Modifications |
None |
Ncbi Official Symbol |
PEBP1 |
Ncbi Gene Id # |
5037 |
User Note |
Optimal dilutions for each application to be determined by the researcher. |
Ncbi Official Symbol |
PEBP1 |
Ncbi Official Full Name |
phosphatidylethanolamine binding protein 1 |
Ncbi Organism |
Homo sapiens |
Swissprot # |
P30086 |
Background |
This gene encodes a member of the phosphatidylethanolamine-binding family of proteins and has been shown to modulate multiple signaling pathways, including the MAP kinase (MAPK), NF-kappa B, and glycogen synthase kinase-3 (GSK-3) signaling pathways. The encoded protein can be further processed to form a smaller cleavage product, hippocampal cholinergic neurostimulating peptide (HCNP), which may be involved in neural development. This gene has been implicated in numerous human cancers and may act as a metastasis suppressor gene. Multiple pseudogenes of this gene have been identified in the genome. |
1st Image Caption |
Western blot analysis of extracts of various cell lines, using PEBP1 antibody (13-208) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s. |
2nd Image Caption |
Immunofluorescence analysis of HeLa cells using PEBP1 antibody (13-208). Blue: DAPI for nuclear staining. |
3rd Image Caption |
Immunoprecipitation analysis of 150ug extracts of MCF-7 cells using 3ug PEBP1 antibody (13-208). Western blot was performed from the immunoprecipitate using PEBP1 antibody (13-208) at a dilition of 1:500. |
Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.
All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.