Comparison

PEBP1 Antibody

Item no. PRS-13-208-50uL
Manufacturer ProSci
Amount 50 uL
Category
Type Antibody Primary
Format Liquid
Applications WB, IF, IP
Specific against Human, Mouse, Rat
Host Rabbit
ECLASS 10.1 32160702
ECLASS 11.0 32160702
UNSPSC 12352203
Alias PEBP1, HCNP, PBP, PEBP, RKIP, HCNPpp, PEBP-1, HEL-210, HEL-S-34
Available
Shipping
blue ice or RT
By Research Area
Cancer, Cell Cycle, Neuroscience
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 1-187 of human PEBP1 (NP_002558.1).
Applications
WB: 1:500 - 1:2000

IF: 1:50 - 1:200

IP: 1:20 - 1:50
Positive Control 1
BT-474
Positive Control 2
293T
Positive Control 3
22Rv1
Positive Control 4
A-549
Positive Control 5
PC-3
Positive Control 6
MCF7
Predicted Molecular Weight
Observed: 22kDa
Purification
Affinity purification
Clonality
Polyclonal
Isotype
IgG
Conjugate
Unconjugated
Buffer
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Storage Conditions
Store at -20˚ C. Avoid freeze / thaw cycles.
Disclaimer
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Modifications
None
Ncbi Official Symbol
PEBP1
Ncbi Gene Id #
5037
User Note
Optimal dilutions for each application to be determined by the researcher.
Ncbi Official Symbol
PEBP1
Ncbi Official Full Name
phosphatidylethanolamine binding protein 1
Ncbi Organism
Homo sapiens
Swissprot #
P30086
Background
This gene encodes a member of the phosphatidylethanolamine-binding family of proteins and has been shown to modulate multiple signaling pathways, including the MAP kinase (MAPK), NF-kappa B, and glycogen synthase kinase-3 (GSK-3) signaling pathways. The encoded protein can be further processed to form a smaller cleavage product, hippocampal cholinergic neurostimulating peptide (HCNP), which may be involved in neural development. This gene has been implicated in numerous human cancers and may act as a metastasis suppressor gene. Multiple pseudogenes of this gene have been identified in the genome.
1st Image Caption
Western blot analysis of extracts of various cell lines, using PEBP1 antibody (13-208) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 10s.
2nd Image Caption
Immunofluorescence analysis of HeLa cells using PEBP1 antibody (13-208). Blue: DAPI for nuclear staining.
3rd Image Caption
Immunoprecipitation analysis of 150ug extracts of MCF-7 cells using 3ug PEBP1 antibody (13-208). Western blot was performed from the immunoprecipitate using PEBP1 antibody (13-208) at a dilition of 1:500.

Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.

All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.

Amount: 50 uL
Available: In stock
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