Item no. |
PRS-13-182-100uL |
Manufacturer |
ProSci
|
Amount |
100 uL |
Category |
|
Type |
Antibody Primary |
Format |
Liquid |
Applications |
WB, IF, IHC |
Specific against |
Human |
Host |
Mouse |
ECLASS 10.1 |
32160702 |
ECLASS 11.0 |
32160702 |
UNSPSC |
12352203 |
Alias |
APEX1, APE, APX, APE1, APEN, APEX, HAP1, REF1 |
Available |
|
Shipping |
blue ice or RT |
By Research Area |
Transcription |
Immunogen |
A synthetic peptide of human APEX1 |
Applications |
WB: 1:500 - 1:1000
IHC: 1:20 - 1:50
IF: 1:20 - 1:50 |
Positive Control 1 |
MCF7 |
Predicted Molecular Weight |
Observed: 36kDa |
Purification |
Affinity purification |
Clonality |
Monoclonal |
Clone |
[B4D3] |
Isotype |
IgG |
Conjugate |
Unconjugated |
Buffer |
PBS with 0.02% sodium azide, pH7.3. |
Storage Conditions |
Store at 4˚ C. Avoid freeze / thaw cycles. |
Disclaimer |
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only. |
Modifications |
None |
Ncbi Official Symbol |
APEX1 |
Ncbi Gene Id # |
328 |
User Note |
Optimal dilutions for each application to be determined by the researcher. |
Ncbi Official Symbol |
APEX1 |
Ncbi Official Full Name |
APEX nuclease (multifunctional DNA repair enzyme) 1 |
Ncbi Organism |
Homo sapiens |
Swissprot # |
P27695 |
Background |
Apurinic/apyrimidinic (AP) sites occur frequently in DNA molecules by spontaneous hydrolysis, by DNA damaging agents or by DNA glycosylases that remove specific abnormal bases. AP sites are pre-mutagenic lesions that can prevent normal DNA replication so the cell contains systems to identify and repair such sites. Class II AP endonucleases cleave the phosphodiester backbone 5' to the AP site. This gene encodes the major AP endonuclease in human cells. Splice variants have been found for this gene; all encode the same protein. |
1st Image Caption |
Western blot analysis of extracts of MCF-7 cells, using APEX1 antibody (13-182). Secondary antibody: HRP Goat Anti-Mouse IgG (H+L) (AS003) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. |
Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.
All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.