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APEX1 Antibody [B4D3]

APEX1 Antibody [B4D3]

Item no.	PRS-13-182-100uL
Manufacturer	ProSci
Amount	100 uL
Category	Neuroscience Molecular Targets for Neuroscience Antibodies Immunohistochemistry Western Blot Immunofluorescence Primary Antibodies
Type	Antibody Primary
Format	Liquid
Applications	WB, IF, IHC
Specific against	Human
Host	Mouse
ECLASS 10.1	32160702
ECLASS 11.0	32160702
UNSPSC	12352203
Alias	APEX1, APE, APX, APE1, APEN, APEX, HAP1, REF1
Available
Shipping	blue ice or RT
By Research Area	Transcription
Immunogen	A synthetic peptide of human APEX1
Applications	WB: 1:500 - 1:1000 IHC: 1:20 - 1:50 IF: 1:20 - 1:50
Positive Control 1	MCF7
Predicted Molecular Weight	Observed: 36kDa
Purification	Affinity purification
Clonality	Monoclonal
Clone	[B4D3]
Isotype	IgG
Conjugate	Unconjugated
Buffer	PBS with 0.02% sodium azide, pH7.3.
Storage Conditions	Store at 4˚ C. Avoid freeze / thaw cycles.
Disclaimer	Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Modifications	None
Ncbi Official Symbol	APEX1
Ncbi Gene Id #	328
User Note	Optimal dilutions for each application to be determined by the researcher.
Ncbi Official Symbol	APEX1
Ncbi Official Full Name	APEX nuclease (multifunctional DNA repair enzyme) 1
Ncbi Organism	Homo sapiens
Swissprot #	P27695
Background	Apurinic/apyrimidinic (AP) sites occur frequently in DNA molecules by spontaneous hydrolysis, by DNA damaging agents or by DNA glycosylases that remove specific abnormal bases. AP sites are pre-mutagenic lesions that can prevent normal DNA replication so the cell contains systems to identify and repair such sites. Class II AP endonucleases cleave the phosphodiester backbone 5' to the AP site. This gene encodes the major AP endonuclease in human cells. Splice variants have been found for this gene; all encode the same protein.
1st Image Caption	Western blot analysis of extracts of MCF-7 cells, using APEX1 antibody (13-182). Secondary antibody: HRP Goat Anti-Mouse IgG (H+L) (AS003) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST.

Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.

All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.

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Amount: 100 uL

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