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IKBKE Antibody

IKBKE Antibody

Item no.	PRS-13-103-50uL
Manufacturer	ProSci
Amount	50 uL
Category	Oncology Antibodies By Cell Type Carcinoma Immunohistochemistry Immunoprecipitation Western Blot Immunofluorescence Primary Antibodies
Type	Antibody Primary
Format	Liquid
Applications	WB, IF, IP, IHC
Specific against	Human, Mouse, Rat
Host	Rabbit
ECLASS 10.1	32160702
ECLASS 11.0	32160702
UNSPSC	12352203
Alias	IKK epsilon Antibody: IKKE, IKKI, IKK-E, IKK-i, IKKE, KIAA0151, Inhibitor of nuclear factor kappa-B kinase subunit epsilon, Inducible I kappa-B kinase, I-kappa-B kinase epsilon
Available
Shipping	blue ice or RT
By Research Area	Apoptosis, Cancer, Cell Cycle, Immunology, Innate Immunity, Signal Transduction
Immunogen	Recombinant fusion protein containing a sequence corresponding to amino acids 495-716 of human IKBKE (NP_054721.1).
Applications	WB: 1:500 - 1:2000 IHC: 1:50 - 1:200 IF: 1:50 - 1:200 IP: 1:50 - 1:200
Positive Control 1	SW480
Positive Control 2	DU145
Positive Control 3	MCF7
Positive Control 4	Mouse liver
Predicted Molecular Weight	Observed: 93kDa
Purification	Affinity purification
Clonality	Polyclonal
Isotype	IgG
Conjugate	Unconjugated
Buffer	PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Storage Conditions	Store at -20˚ C. Avoid freeze / thaw cycles.
Disclaimer	Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Modifications	None
Ncbi Official Symbol	IKBKE
Ncbi Gene Id #	9641
User Note	Optimal dilutions for each application to be determined by the researcher.
Ncbi Official Symbol	IKBKE
Ncbi Official Full Name	inhibitor of kappa light polypeptide gene enhancer in B-cells, kinase epsilon
Ncbi Organism	Homo sapiens
Swissprot #	Q14164
1st Image Caption	Western blot analysis of extracts of various cell lines, using IKBKE antibody (13-103). Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST.
2nd Image Caption	Immunohistochemistry of paraffin-embedded human colon carcinoma using IKBKE antibody (13-103) at dilution of 1:200 (40x lens).
3rd Image Caption	Immunohistochemistry of paraffin-embedded rat spleen using IKBKE antibody (13-103) at dilution of 1:200 (40x lens).
4th Image Caption	Immunohistochemistry of paraffin-embedded mouse spleen using IKBKE antibody (13-103) at dilution of 1:200 (40x lens).
5th Image Caption	Immunofluorescence analysis of NIH/3T3 cells using IKBKE antibody (13-103) at dilution of 1:100. Blue: DAPI for nuclear staining.

Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.

All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.

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Amount: 50 uL

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Delivery expected until 10/4/2024

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