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CDK1 Antibody

CDK1 Antibody

Item no.	PRS-13-084-50uL
Manufacturer	ProSci
Amount	50 uL
Category	Oncology Antibodies Immunohistochemistry Western Blot Immunofluorescence Primary Antibodies By Organ Lung Cancer
Type	Antibody Primary
Format	Liquid
Applications	WB, IF, IHC
Specific against	Human, Mouse, Rat
Host	Rabbit
ECLASS 10.1	32160702
ECLASS 11.0	32160702
UNSPSC	12352203
Alias	CDC2, CDC28A, CDK1, DKFZp686L20222, MGC111195, CDC2, P34CDC2
Available
Shipping	blue ice or RT
By Research Area	Apoptosis, Cell Cycle, Immunology, Neuroscience, Signal Transduction
Immunogen	A synthetic peptide corresponding to a sequence within amino acids 208-258 of human CDK1 (NP_001777.1).
Applications	WB: 1:500 - 1:2000 IHC: 1:50 - 1:100 IF: 1:50 - 1:200
Positive Control 1	HL-60
Positive Control 2	HeLa
Positive Control 3	293T
Positive Control 4	THP-1
Positive Control 5	BT-474
Positive Control 6	Mouse thymus
Predicted Molecular Weight	Observed: 34kDa
Purification	Affinity purification
Clonality	Polyclonal
Isotype	IgG
Conjugate	Unconjugated
Buffer	PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Storage Conditions	Store at -20˚ C. Avoid freeze / thaw cycles.
Disclaimer	Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Modifications	None
Ncbi Official Symbol	CDK1
Ncbi Gene Id #	983
User Note	Optimal dilutions for each application to be determined by the researcher.
Ncbi Official Symbol	CDK1
Ncbi Official Full Name	cyclin-dependent kinase 1
Ncbi Organism	Homo sapiens
Swissprot #	P06493
Background	The protein encoded by this gene is a member of the Ser/Thr protein kinase family. This protein is a catalytic subunit of the highly conserved protein kinase complex known as M-phase promoting factor (MPF), which is essential for G1/S and G2/M phase transitions of eukaryotic cell cycle. Mitotic cyclins stably associate with this protein and function as regulatory subunits. The kinase activity of this protein is controlled by cyclin accumulation and destruction through the cell cycle. The phosphorylation and dephosphorylation of this protein also play important regulatory roles in cell cycle control. Alternatively spliced transcript variants encoding different isoforms have been found for this gene.
1st Image Caption	Western blot analysis of extracts of various cell lines, using CDK1 antibody (13-084) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST.
2nd Image Caption	Immunohistochemistry of paraffin-embedded rat brain using CDK1 antibody (13-084) at dilution of 1:100 (40x lens).
3rd Image Caption	Immunohistochemistry of paraffin-embedded mouse spinal cord using CDK1 antibody (13-084) at dilution of 1:100 (40x lens).
4th Image Caption	Immunohistochemistry of paraffin-embedded mouse brain using CDK1 antibody (13-084) at dilution of 1:100 (40x lens).
5th Image Caption	Immunofluorescence analysis of A549 cells using CDK1 antibody (13-084). Blue: DAPI for nuclear staining.

Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.

All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.

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