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BMI1 Antibody

BMI1 Antibody

Item no.	PRS-13-080-50uL
Manufacturer	ProSci
Amount	50 uL
Category	Oncology Neuroscience Molecular Targets for Neuroscience Antibodies By Cell Type Carcinoma Hematology Leukemia Immunoprecipitation Western Blot Primary Antibodies
Type	Antibody Primary
Format	Liquid
Applications	WB, IP
Specific against	Human
Host	Rabbit
ECLASS 10.1	32160702
ECLASS 11.0	32160702
UNSPSC	12352203
Alias	BMI-1 Antibody: PCGF4, RNF51, FLVI2/BMI1, PCGF4, Polycomb complex protein BMI-1, Polycomb group RING finger protein 4
Available
Shipping	blue ice or RT
By Research Area	Cell Cycle
Immunogen	Recombinant fusion protein containing a sequence corresponding to amino acids 120-326 of human BMI1 (NP_005171.4).
Applications	WB: 1:500 - 1:2000 IP: 1:50 - 1:100
Positive Control 1	HT-1080
Positive Control 2	K-562
Positive Control 3	U-937
Predicted Molecular Weight	Observed: 42kDa
Purification	Affinity purification
Clonality	Polyclonal
Isotype	IgG
Conjugate	Unconjugated
Buffer	PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Storage Conditions	Store at -20˚ C. Avoid freeze / thaw cycles.
Disclaimer	Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Modifications	None
Ncbi Official Symbol	BMI1
Ncbi Gene Id #	648
User Note	Optimal dilutions for each application to be determined by the researcher.
Ncbi Official Symbol	BMI1
Ncbi Official Full Name	BMI1 polycomb ring finger oncogene
Ncbi Organism	Homo sapiens
Swissprot #	P35226
Background	This gene encodes a ring finger protein that is major component of the polycomb group complex 1 (PRC1). This complex functions through chromatin remodeling as an essential epigenetic repressor of multiple regulatory genes involved in embryonic development and self-renewal in somatic stem cells. This protein also plays a central role in DNA damage repair. This gene is an oncogene and aberrant expression is associated with numerous cancers and is associated with resistance to certain chemotherapies. A pseudogene of this gene is found on chromosome X. Read-through transcription also exists between this gene and the upstream COMM domain containing 3 (COMMD3) gene.
1st Image Caption	Western blot analysis of extracts of various cell lines, using BMI1 antibody (13-080) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.
2nd Image Caption	Immunoprecipitation analysis of 200ug extracts of K-562 cells, using 3 ug BMI1 antibody (13-080). Western blot was performed from the immunoprecipitate using BMI1 antibody (13-080) at a dilition of 1:1000.

Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.

All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.

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Amount: 50 uL

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Delivery expected until 10/4/2024

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