Item no. |
PRS-13-080-50uL |
Manufacturer |
ProSci
|
Amount |
50 uL |
Category |
|
Type |
Antibody Primary |
Format |
Liquid |
Applications |
WB, IP |
Specific against |
Human |
Host |
Rabbit |
ECLASS 10.1 |
32160702 |
ECLASS 11.0 |
32160702 |
UNSPSC |
12352203 |
Alias |
BMI-1 Antibody: PCGF4, RNF51, FLVI2/BMI1, PCGF4, Polycomb complex protein BMI-1, Polycomb group RING finger protein 4 |
Available |
|
Shipping |
blue ice or RT |
By Research Area |
Cell Cycle |
Immunogen |
Recombinant fusion protein containing a sequence corresponding to amino acids 120-326 of human BMI1 (NP_005171.4). |
Applications |
WB: 1:500 - 1:2000
IP: 1:50 - 1:100 |
Positive Control 1 |
HT-1080 |
Positive Control 2 |
K-562 |
Positive Control 3 |
U-937 |
Predicted Molecular Weight |
Observed: 42kDa |
Purification |
Affinity purification |
Clonality |
Polyclonal |
Isotype |
IgG |
Conjugate |
Unconjugated |
Buffer |
PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
Storage Conditions |
Store at -20˚ C. Avoid freeze / thaw cycles. |
Disclaimer |
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only. |
Modifications |
None |
Ncbi Official Symbol |
BMI1 |
Ncbi Gene Id # |
648 |
User Note |
Optimal dilutions for each application to be determined by the researcher. |
Ncbi Official Symbol |
BMI1 |
Ncbi Official Full Name |
BMI1 polycomb ring finger oncogene |
Ncbi Organism |
Homo sapiens |
Swissprot # |
P35226 |
Background |
This gene encodes a ring finger protein that is major component of the polycomb group complex 1 (PRC1). This complex functions through chromatin remodeling as an essential epigenetic repressor of multiple regulatory genes involved in embryonic development and self-renewal in somatic stem cells. This protein also plays a central role in DNA damage repair. This gene is an oncogene and aberrant expression is associated with numerous cancers and is associated with resistance to certain chemotherapies. A pseudogene of this gene is found on chromosome X. Read-through transcription also exists between this gene and the upstream COMM domain containing 3 (COMMD3) gene. |
1st Image Caption |
Western blot analysis of extracts of various cell lines, using BMI1 antibody (13-080) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s. |
2nd Image Caption |
Immunoprecipitation analysis of 200ug extracts of K-562 cells, using 3 ug BMI1 antibody (13-080). Western blot was performed from the immunoprecipitate using BMI1 antibody (13-080) at a dilition of 1:1000. |
Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.
All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.