Item no. |
PRS-1120-0.02mg |
Manufacturer |
ProSci
|
Amount |
0.02 mg |
Category |
|
Type |
Antibody Polyclonal |
Format |
Liquid |
Applications |
WB, IF, ELISA |
Specific against |
Human, Mouse, Rat |
Host |
Rabbit |
ECLASS 10.1 |
32160702 |
ECLASS 11.0 |
32160702 |
UNSPSC |
12352203 |
Alias |
DR3 Antibody: DR3, TR3, DDR3, LARD, APO-3, TRAMP, WSL-1, WSL-LR, TNFRSF12, APO3, DR3, WSL, WSL1, UNQ455/PRO779, Tumor necrosis factor receptor superfamily member 25, Apo-3 |
Available |
|
Shipping |
blue ice or RT |
By Research Area |
Apoptosis |
Immunogen |
DR3 antibody was raised against a 20 amino acid peptide near the carboxy terminus of human DR3.
The immunogen is located within the last 50 amino acids of DR3. |
Applications |
DR3 antibody can be used for Western blot 0.5 μ g/mL. A 59 kDa band should be detected.
Antibody validated: Western Blot in human samples and Immunofluorescence in human samples. All other applications and species not yet tested. |
Specificity |
No cross reaction to other death receptors. |
Positive Control 1 |
Cat. No. 1205 - Jurkat Cell Lysate |
Predicted Molecular Weight |
59 kDa |
Purification |
DR3 Antibody is Antibody is DEAE purified. |
Clonality |
Polyclonal |
Isotype |
IgG |
Conjugate |
Unconjugated |
Buffer |
DR3 Antibody is supplied in PBS containing 0.02% sodium azide. |
Concentration |
1 mg/ml |
Storage Conditions |
DR3 antibody can be stored at 4˚ C for three months and -20˚ C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
Related Products |
Cat. No. 1120P – DR3 Peptide |
Disclaimer |
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only. |
Modifications |
None |
Ncbi Official Symbol |
TNFRSF25 |
Accession # |
AAQ88676 |
Protein Gi # |
37181738 |
Ncbi Gene Id # |
8718 |
User Note |
Optimal dilutions for each application to be determined by the researcher. |
Ncbi Official Symbol |
TNFRSF25 |
Ncbi Official Full Name |
tumor necrosis factor receptor superfamily, member 25 |
Ncbi Organism |
Homo sapiens |
Swissprot # |
Q93038 |
Background |
DR3 Antibody: Apoptosis, or programmed cell death, occurs during normal cellular differentiation and development of multicellular organisms. Apoptosis is induced by certain cytokines including TNF and Fas ligand of the TNF family through their death domain containing receptors, TNFR1 and Fas. A novel cell death receptor was recently identified by several groups independently and designated DR3, Wsl-1, Apo-3, TRAMP and LARD1-5. The ligand for this novel cell death receptor has not yet been defined. DR3 is highly expressed in the tissues enriched in lymphocytes including PBL, thymus and spleen. Like TNFR1, DR3 induces apoptosis and NF-κ B activation. |
Background References 1 |
Chinnaiyan AM; O'Rourke K; Yu GL; Lyons RH; Garg M; Duan DR; Xing L; Gentz R; Ni J; Dixit VM. Science, 1996; 274:990- |
Background References 2 |
Kitson J; Raven T; Jiang YP; Goeddel DV; Giles KM; Pun KT; Grinham CJ; Brown R; Farrow SN. Nature, 1996; 384:372-5. |
Background References 3 |
Marsters SA; Sheridan JP; Donahue CJ; Pitti RM; Gray CL; Goddard AD; Bauer KD; Ashkenazi A. Curr Biol, 1996; 6:1669-76. |
Background References 4 |
Bodmer JL; Burns K; Schneider P; Hofmann K; Steiner V; Thome M; Bornand T; Hahne M; Schroter M; Becker K; et al. Immunity, 1997; 6:79-88. |
1st Image Caption |
Western blot analysis of DR3 in Jurkat total cell lysate with DR3 antibody at 1:500 dilution. |
2nd Image Caption |
Immunofluorescence of DR3 in Jurkat cells with DR3 antibody at 20 μ g/mL.
Green: DR3 Antibody (1120) Blue: DAPI staining |
Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.
All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.