Item no. |
NKM-ATGA0593-50ul |
Manufacturer |
NKMAX
|
Amount |
50ul |
Category |
|
Type |
Antibody Primary |
Format |
Liquid in. Phosphate-Buffered Saline (pH 7.4) with 0.02% Sodium Azide, 10% glycerol |
Applications |
WB, ELISA |
Clone |
AT14H8 |
Specific against |
other |
Host |
Mouse |
Isotype |
IgG2b kappa |
Conjugate/Tag |
Unconjugated |
Citations |
Characterization of novel monoclonal antibodies against MERS-coronavirus spike protein{https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7114870/} |
NCBI |
AFS88936 |
ECLASS 10.1 |
32160702 |
ECLASS 11.0 |
32160702 |
UNSPSC |
12352203 |
Alias |
Middle East respiratory syndrome coronavirus, Human betacoronavirus 2c EMC/2012, MERS-CoV, MERS, MERS-CoV S2 P, Spike2 glycoprotein, S2 glycoprotein, S2, Spike S2 Subunit protein, S2 Subunit |
Available |
|
Ab Purification Comment |
By protein-A affinity chromatography |
Ab Usage |
The antibody has been tested by ELISA analysis to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results. |
Additional Comment Only |
S2-14H8 mAb, which showed binding affinity to S2, showed partial neutralizing effect. (Virus Res. 2020 Mar 278:197863) |
Additional Info Comment |
ELISA: MERS Spike S2 Antibody (1ug/ml) specifically recognizes MERS Spike and Spike S2 recombinant protein, but not interacted MERS Spike S1 and RBD recombinant protein in ELISA. |
Antigen Species |
MERS-CoV |
Clonality Mono VS Poly |
Monoclonal |
Concentration |
1mg/ml (determined by BCA assay) |
Description |
MERS-CoV, which causes the Middles East Respiratory Syndrome (MERS), belongs to a family of viruses known as coronaviruses. MERS-CoV was first identified in the Kingdom of Saudi Arabia in 2012, which is a single and positive stranded RNA virus. Dromedary camels are widely considered as the source of the transmission of MERS-CoV. The rate of human transmission among household contacts of MERS patients has been approximately 5 % based on serological analysis. MERS-CoV has four structural proteins, known as the S (spike), E (envelope), M (membrane), and N (nucleocapsid) proteins. The spike protein, responsible for allowing the virus to attach to and fuse with the membrane of a host cell and is a large type I transmembrane protein containing two subunits, S1 and S2. S1 mainly contains a receptor binding domain (RBD), which is responsible for recognizing the cell surface receptor. S2 contains basic elements needed for the membrane fusion. MERS-CoV S mediates viral attachment and fusion to human cells via human cellular receptor DPP4, also known as CD26. The S protein plays key parts in the induction of neutralizing-antibody and T-cell responses, as well as protective immunity. |
Gene Category |
Virus |
General References |
Junghyun Goo., et al. (2020) Virus Res. 278:197863. ; ; Yan-Hua Li., et al. (2019) Engineering. 5:940-947.; ; Lingshu Wang., et al. (2018) J Virol. 92:e02002-2017. ; ; Nicolas Papageorgiou., et al. (2016) Acta Crystallogr D Struct Biol. 72:192-202.; ; Xiao-Yan Che., et al. (2004) J Clin Microbiol. 42:2629-2635.; ; |
Immunogen |
Recombinant MERS-CoV Spike S2 Subunit (752-1296aa) purified from Baculovirus |
Product Type Name |
Monoclonal antibody |
Storage |
Can be stored at +2C to +8C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles. |
WB Comment and Pic |
The recombinant proteins (50ng) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-MERS Spike S2 antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.; ; Lane 1.: Recombinant MERS Spike protein (ATGP3979); ; Lane 2.: Recombinant MERS Spike S1 protein (ATGP3980); ; Lane 3.: Recombinant MERS Spike S2 protein (ATGP3981); ; Lane 4.: Recombinant MERS Spike RBD protein (ATGP3982); ; |
Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.
All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.