Item no. |
MCHERRY-0100 |
Manufacturer |
Aves Labs
|
Amount |
500 uL |
Quantity options |
100 uL
500 uL
|
Category |
|
Type |
Antibody Primary |
Format |
Liquid |
Applications |
WB, ICC |
Specific against |
other |
Host |
Chicken |
Isotype |
IgY |
Conjugate/Tag |
Unconjugated |
ECLASS 10.1 |
32160702 |
ECLASS 11.0 |
32160702 |
UNSPSC |
12352203 |
Shipping condition |
Cool pack |
Available |
|
Manufacturer - Type |
Primary Antibody |
Manufacturer - Category |
Anti-Tag Antibodies |
Manufacturer - Targets |
mCherry |
Country of Origin |
United States |
Storage Conditions |
Store at 4°C in the dark. Under these conditions, the antibodies should have a shelf life of at least twelve months, provided they remain sterile. For longer term storage, aliquot and freeze to avoid freeze-thaw of the antibody. |
Molecular Weight |
27 kDa for native monomer |
Concentration |
0.2 mg/mL |
Buffer |
Phosphate-buffered (10 mM) isotonic (0.9%, w/v) saline (“PBS, " pH 7.2) with sodium azide (0.02%, w/v) added as a preservative. |
Quality Control |
Each new lot of this antibody is tested by Western blot on an mCherry overexpression cell lysate. |
Description |
Our Anti-mCherry chicken polyclonal primary antibody from Aves Labs is produced in-house. It detects mCherry, and is affinity purified. It is great for use in ICC, WB. |
Manufacturer - Statement |
We produce our Anti-mCherry chicken polyclonal primary antibody. It is great in ICC, WB and is affinity purified. |
Target Description |
mCherry is a red fluorescent protein derived from DsRed which is isolated from disc corals and related to GFP (green fluorescent protein) (Shaner et al., 2004). Fluorescent proteins are commonly used to tag proteins in order to localize their expression in both cultured cells and in vivo. Antibodies against fluorescent proteins such as mCherry are especially useful when amplification of the fluorescent signal is necessary or desired. Our chicken anti-mCherry antibody is a highly sensitive reagent for detection of mCherry by western blot, immunocytochemistry, and immunohistochemistry. |
Antibody Registry ID (RRID) |
AB_2910557 |
Clonality |
Polyclonal |
Dilution Range ICC |
1:100-1:500 |
Dilution Range Western Blot |
1:500-1:1000 |
Immunogen |
Purified recombinant mCherry (Uniprot X5DSL3) expressed in E.Coli |
Manufacturer - Form |
Affinity purified |
Expiration |
12 months from receipt of product |
Usage Statement |
These antibodies are to be used as research laboratory reagents and are not for use as diagnostic or therapeutic reagents in humans. |
Production Notes |
Eggs from hens hyperimmunized with target were used to prepare an IgY fraction which was then subjected to antigen-specific affinity purification |
Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.
All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.