Anti-Mouse TCR beta chain - DyLight 594

Antibody Details

Product Details

Reactivity Species

Mouse

Host Species

Armenian Hamster

Immunogen

Affinity purified TCR from mouse DO-11.10 cells

Product Concentration

0.2 mg/ml

Formulation

This DyLight® 594 conjugate is formulated in 0.01 M phosphate buffered saline (150 mM NaCl) PBS pH 7.4, 1% BSA and 0.09% sodium azide as a preservative.

Storage and Handling

This DyLight® 594 conjugate is stable when stored at 2-8°C. Do not freeze.

Country of Origin

USA

Shipping

Next Day 2-8°C

Excitation Laser

Red Laser (590 nm)

RRID

AB_2832112

Applications and Recommended Usage?
Quality Tested by Leinco

FC The suggested concentration for this TCR β chain (clone H57-597) antibody for staining cells in flow cytometry is ≤ 0.25 μg per 10⁶ cells in a volume of 100 μl. Titration of the reagent is recommended for optimal performance for each application.

Other Applications Reported In Literature ?

IHC FF

Each investigator should determine their own optimal working dilution for specific applications. See directions on lot specific datasheets, as information may periodically change.

Description

Specificity

Clone H57-597 recognizes an epitope on the constant region of mouse TCRβ. Clone H57-597 does not cross-react with γδ T cells.

Antigen Distribution

TCRβ is expressed on αβ thymocytes, peripheral T cells, and NKT cells.

Background

TCRβ antibody, clone H57-597, recognizes the beta chain of the T cell receptor (TCRβ), expressed on αβ thymocytes and T lymphocytes, as well as natural killer T (NKT) cells¹. TCRβ is a transmembrane protein that consists of an amino-terminal variable region, a constant region, and a stalk segment covalently linked to the TCR alpha chain (TCRα) via a disulfide bond². TCRα/β does not contain signaling domains and is instead noncovalently associated with the CD3 complex (TCR-CD3), essential for TCR signaling^{3, 4}. TCRα/β recognizes antigens bound to major histocompatibility complex (MHC) molecules, resulting in phosphorylation of CD3 ITAMs and subsequent T cell activation, proliferation, and survival^{5, 6}.

Antigen Details

Protein

TCR beta

Ligand/Receptor

Peptide bound-MHC class I and II

Function

Antigen recognition, T cell activation

PubMed

TCR beta

NCBI Gene Bank ID

21577

UniProt.org

Information on Uniprot.org

References & Citations

1. Krijgsman D, et al. (2018) Front Immunol. 9:367

2. Clevers H, et al. (1988) Annu Rev Immunol. 6:629-62

3. Kuhns MS, Badgandi HB. (2012) Immunol Rev. 250(1):120-43

4. Wucherpfennig KW, et al. (2010) Cold Spring Harb Perspect Biol. 2(4):a005140

5. Samelson LE. (2002) Annu Rev Immunol. 20:371-94

6. Borroto A, et al. (2014) Immunol Lett. 161(1):113-7

Technical Protocols