Anti-Human TRAIL R3 - Biotin

Antibody Details

Product Details

Reactivity Species

Human

Host Species

Goat

Immunogen

Purified Recombinant Human TRAIL R3 (Accession # O14798)

Formulation

This biotinylated antigen affinity purified polyclonal antibody has been 0.2 µm filtered and lyophilized from modified Dulbecco’s phosphate buffered saline (1X PBS) pH 7.2 – 7.3 containing 50 µg of bovine serum albumin per µg of antibody with no calcium, magnesium, or preservatives present.

Storage and Handling

The lyophilized, biotinylated antigen affinity purified polyclonal antibody can be stored desiccated at -20°C to -70°C for up to twelve months from date of receipt. The reconstituted biotin conjugate can be stored for at least four weeks at 2-8°C. For long-term storage of the reconstituted conjugate, aseptically aliquot into working volumes and store at -20°C to -70°C in a manual defrost freezer. Avoid Repeated Freeze Thaw Cycles. No detectable loss of activity was observed after six months.

Country of Origin

USA

Shipping

Next Day Ambient

RRID

AB_2831996

Applications and Recommended Usage?
Quality Tested by Leinco

Western Blotting: To detect Human TRAIL R3 this biotin conjugate can be used at a concentration of 0.1 - 0.2 µg/ml. This biotin conjugate should be used in conjunction with compatible second-step reagents such as PN:A106 and a chromogenic substrate such as PN:T343. The detection limit for Human TRAIL R3 is 2 ng/lane under either reducing or non-reducing conditions. The sensitivity of detection may increase up to 50 fold when a chemiluminescent substrate is used. A suitable Western blotting control is PN:T402.
ELISA Sandwich Assay: This antibody can be used as the detection antibody in a sandwich ELISA at a concentration of approximately 0.1-0.4 µg/mL when used in conjunction with as the capture antibody at 2 µg/ml.

Each investigator should determine their own optimal working dilution for specific applications. See directions on lot specific datasheets, as information may periodically change.

Description

Specificity

Goat Anti-Human TNF-Related Apoptosis-Inducing Ligand Receptor 3 (TRAIL R3) recognizes Human TRAIL R3. This antigen affinity purified polyclonal antibody was purified using a proprietary chromatographic technique that includes covalently immobilizing the antigen proteins or peptides to agarose based beads. This purification method enhances specificity, reduces nonspecific binding of extraneous IgG and provides you with the most reliable reagent available for your early discovery research.

Background

TNF-related apoptosis-inducing ligand receptor 3 (TRAIL R3) is an antagonist decoy receptor¹ that can inhibit TRAIL-induced apoptosis.² TRAIL R3 is a plasma membrane-bound protein capable of high affinity interaction with the TRAIL ligand.³

Antigen Details

Protein

TRAIL R3

PubMed

TRAIL R3

NCBI Gene Bank ID

8794

UniProt.org

More Information on Uniprot.org

References & Citations

1. Fornace Jr., AJ. et al. (1999) Oncogene 18: 4153

2. Büchler, MW. et al. (2001) Anticancer Res. 21: 3153

3. Smith, CA. et al. (1997) J Exp Med. 186: 1165

Technical Protocols