Antibody Details
Product Details
Reactivity Species
Human
Host Species
Goat
Immunogen
Purified Recombinant Human Tie-2 (> 98%)
Formulation
This antigen affinity purified polyclonal antibody has been 0.2 µm filtered and lyophilized from modified Dulbecco’s phosphate buffered saline (1X PBS) pH 7.2 – 7.3 containing 5.0% w/v trehalose with no calcium, magnesium, or preservatives present.
Storage and Handling
The lyophilized antigen affinity purified polyclonal antibody can be stored desiccated at -20°C to -70°C for twelve months from date of receipt. The reconstituted antibody can be stored for at least four weeks at 2-8°C. For long-term storage of the reconstituted antibody, aseptically aliquot into working volumes and store at -20°C to -70°C in a manual defrost freezer. Avoid Repeated Freeze Thaw Cycles. No detectable loss of activity was observed after six months.
Country of Origin
USA
Shipping
Next Day Ambient
RRID
Applications and Recommended Usage?
Quality Tested by Leinco
Western Blotting: To detect Human Tie-2 this polyclonal antibody can be used at a concentration of 0.1-0.2 µg/ml. This polyclonal antibody should be used in conjunction with compatible second-step reagents such as PN:G505 and a chromogenic substrate such as PN:T343. The detection limit for Human Tie-2 is 1 ng/lane under either reducing or non-reducing conditions. The sensitivity of detection may increase up to 50 fold when a chemiluminescent substrate is used. A suitable Western blotting control is PN:T388.
Other Applications Reported In Literature ?
IHC (NBF/Par.): This antibody should give satisfactory staining results when used at a concentration of 3-10 µg/ml for 5-15 µm thick sections. The recommended secondary antibody for IHC is PN:G505. For chromogenic detection with high signal and low background use PN:D100 or PN:K107.
Blocking: Neutralization of receptor-ligand interaction - Approximately 5-10 μg/mlof this antibody will block 50% of the binding of 40 ng/ml of recombinant human Angiopoietin-2 to immobilized recombinant human Tie-2/Fc chimera (100 μl of a 4 μg/ml solution was coated in each well) in a functional ELISA assay.
Each investigator should determine their own optimal working dilution for specific applications. See directions on lot specific datasheets, as information may periodically change.
Description
Specificity
Goat Anti-Human Tie-2 recognizes Human Tie-2. This antigen affinity purified polyclonal antibody was purified using a proprietary chromatographic technique that includes covalently immobilizing the antigen proteins or peptides to agarose based beads. This purification method enhances specificity, reduces nonspecific binding of extraneous IgG and provides you with the most reliable reagent available for your early discovery research.
Background
Tyrosine kinase with immunoglobulin-like and EGF-like domains 2 (Tie-2) are novel endothelial cell tyrosine kinase receptors that are essential for vascular development and remodeling in the embryo.1 They are expressed almost exclusively in endothelial cells.2 Tie-2 is functional in pericytes and may play an important role in the progression of diabetic retinopathy, by regulating pericyte loss and influencing the activation state and recruitment of pericytes.3 Tie2 has been shown to be up-regulated in psoriasis and in breast cancer tissue and blocking Tie2 action inhibits tumor angiogenesis and tumor growth.
Antigen Details
Protein
Tie-2
PubMed
Tie-2
NCBI Gene Bank ID
7010
UniProt.org
More Information on Uniprot.org
References & Citations
1. Vartikovski, L. et al. (1999) J Vasc Res. 36: 272
2. Peters, KG. et al. (2002) Mol Cell Biol. 22: 1704
3. Boulton, ME. et al. (2008) Invest Ophthalmol Vis Sci 49: 2163
Technical Protocols