Anti-Human Tie-2

Antibody Details

Product Details

Reactivity Species

Human

Host Species

Goat

Immunogen

Purified Recombinant Human Tie-2 (> 98%)

Formulation

This antigen affinity purified polyclonal antibody has been 0.2 µm filtered and lyophilized from modified Dulbecco’s phosphate buffered saline (1X PBS) pH 7.2 – 7.3 containing 5.0% w/v trehalose with no calcium, magnesium, or preservatives present.

Storage and Handling

The lyophilized antigen affinity purified polyclonal antibody can be stored desiccated at -20°C to -70°C for twelve months from date of receipt. The reconstituted antibody can be stored for at least four weeks at 2-8°C. For long-term storage of the reconstituted antibody, aseptically aliquot into working volumes and store at -20°C to -70°C in a manual defrost freezer. Avoid Repeated Freeze Thaw Cycles. No detectable loss of activity was observed after six months.

Country of Origin

USA

Shipping

Next Day Ambient

RRID

AB_2831919

Applications and Recommended Usage?
Quality Tested by Leinco

Western Blotting: To detect Human Tie-2 this polyclonal antibody can be used at a concentration of 0.1-0.2 µg/ml. This polyclonal antibody should be used in conjunction with compatible second-step reagents such as PN:G505 and a chromogenic substrate such as PN:T343. The detection limit for Human Tie-2 is 1 ng/lane under either reducing or non-reducing conditions. The sensitivity of detection may increase up to 50 fold when a chemiluminescent substrate is used. A suitable Western blotting control is PN:T388.

Other Applications Reported In Literature ?

IHC (NBF/Par.): This antibody should give satisfactory staining results when used at a concentration of 3-10 µg/ml for 5-15 µm thick sections. The recommended secondary antibody for IHC is PN:G505. For chromogenic detection with high signal and low background use PN:D100 or PN:K107.
Blocking: Neutralization of receptor-ligand interaction - Approximately 5-10 μg/mlof this antibody will block 50% of the binding of 40 ng/ml of recombinant human Angiopoietin-2 to immobilized recombinant human Tie-2/Fc chimera (100 μl of a 4 μg/ml solution was coated in each well) in a functional ELISA assay.

Each investigator should determine their own optimal working dilution for specific applications. See directions on lot specific datasheets, as information may periodically change.

Description

Specificity

Goat Anti-Human Tie-2 recognizes Human Tie-2. This antigen affinity purified polyclonal antibody was purified using a proprietary chromatographic technique that includes covalently immobilizing the antigen proteins or peptides to agarose based beads. This purification method enhances specificity, reduces nonspecific binding of extraneous IgG and provides you with the most reliable reagent available for your early discovery research.

Background

Tyrosine kinase with immunoglobulin-like and EGF-like domains 2 (Tie-2) are novel endothelial cell tyrosine kinase receptors that are essential for vascular development and remodeling in the embryo.¹ They are expressed almost exclusively in endothelial cells.² Tie-2 is functional in pericytes and may play an important role in the progression of diabetic retinopathy, by regulating pericyte loss and influencing the activation state and recruitment of pericytes.³ Tie2 has been shown to be up-regulated in psoriasis and in breast cancer tissue and blocking Tie2 action inhibits tumor angiogenesis and tumor growth.

Antigen Details

Protein

Tie-2

PubMed

Tie-2

NCBI Gene Bank ID

7010

UniProt.org

More Information on Uniprot.org

References & Citations

1. Vartikovski, L. et al. (1999) J Vasc Res. 36: 272

2. Peters, KG. et al. (2002) Mol Cell Biol. 22: 1704

3. Boulton, ME. et al. (2008) Invest Ophthalmol Vis Sci 49: 2163

Technical Protocols