Anti-Human sTNF RI

Antibody Details

Product Details

Reactivity Species

Human

Host Species

Goat

Immunogen

E. coli-derived human soluble TNF RI

Endotoxin Level

< 0.1 EU/µg as determined by the LAL method

Formulation

This antigen affinity purified polyclonal antibody has been 0.2 µm filtered and lyophilized from modified Dulbecco’s phosphate buffered saline (1X PBS) pH 7.2 – 7.3 containing 5.0% w/v trehalose with no calcium, magnesium, or preservatives present.

Storage and Handling

The lyophilized antigen affinity purified polyclonal antibody can be stored desiccated at -20°C to -70°C for twelve months from date of receipt. The reconstituted antibody can be stored for at least four weeks at 2-8°C. For long-term storage of the reconstituted antibody, aseptically aliquot into working volumes and store at -20°C to -70°C in a manual defrost freezer. Avoid Repeated Freeze Thaw Cycles. No detectable loss of activity was observed after six months.

Country of Origin

USA

Shipping

Next Day Ambient

RRID

AB_2831918

Applications and Recommended Usage?
Quality Tested by Leinco

Western Blotting: To detect Human TNF RI this polyclonal antibody can be used at a concentration of 0.1-0.2 µg/ml. This polyclonal antibody should be used in conjunction with compatible second-step reagents such as PN:G505 and a chromogenic substrate such as PN:T343. The detection limit for Human TNF RI is 1 ng/lane under non-reducing and 25 ng/lane under reducing conditions. The sensitivity of detection may increase up to 50 fold when a chemiluminescent substrate is used. A suitable Western blotting control is PN:T390.
Flow Cytometry: Suitable for use at concentration of 2.5 µg/10⁶ cells.

Other Applications Reported In Literature ?

Immunocytochemistry: Suitable for use at concentration of 5-15 µg/mL.
CyTOF-ready: Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.

Each investigator should determine their own optimal working dilution for specific applications. See directions on lot specific datasheets, as information may periodically change.

Description

Specificity

Goat Anti-Human Soluble Tumor Necrosis Factor Receptor I (TNF RI) recognizes Human TNF RI. This antigen affinity purified polyclonal antibody was purified using a proprietary chromatographic technique that includes covalently immobilizing the antigen proteins or peptides to agarose based beads. This purification method enhances specificity, reduces nonspecific binding of extraneous IgG and provides you with the most reliable reagent available for your early discovery research.

Background

Tumor necrosis factor receptor superfamily, member 1A, (TNFRI) also known as TNFRSF1A or CD120A is the principal receptor through which many of the pro-inflammatory activities of TNF-alpha are mediated.¹ TNFRI contains a death domain through which it interacts with other death-domain proteins to promote cellular responses.² It regulates immune and inflammatory responses.³

Antigen Details

Protein

sTNF RI

PubMed

sTNF RI

NCBI Gene Bank ID

7132

UniProt.org

More Information on Uniprot.org

References & Citations

1. Dana, R. et al. (2000) Arch Ophthalmol. 118: 1666

2. Donner, DB. et al. (2008) J Immunol. 181: 1288

3. Pasparakis, M. et al. (2008) Nat Immunol. 9: 1015

Technical Protocols