Anti-Human TRIM5gamma

Antibody Details

Product Details

Reactivity Species

Human

Host Species

Rabbit

Product Concentration

0.5 mg/ml

Formulation

This polyclonal antibody is formulated in phosphate buffered saline (PBS) pH 7.4 containing 0.02% sodium azide as a preservative.

Storage and Handling

This polyclonal antibody is stable for at least one week when stored at 2-8°C. For long term storage, aliquot in working volumes without diluting and store at –20°C in a manual defrost freezer. Avoid Repeated Freeze Thaw Cycles.

Country of Origin

USA

Shipping

Next Day Ambient

RRID

AB_2831903

Each investigator should determine their own optimal working dilution for specific applications. See directions on lot specific datasheets, as information may periodically change.

Description

Specificity

Rabbit Anti-Human TRIM5γ recognizes Human TRIM5γ. This polyclonal antibody was purified using affinity chromatography.

Background

TRIM5 is a member of a broad family of otherwise unrelated proteins defined by the presence of a tripartite motif containing a RING domain, a B-box type 1, and a B-box type 2, followed by a coiled-coil region.¹ TRIM5 has five alternately spliced isoforms, the longest of which is the variant which also contains a carboxy-terminal B30.2 (SPRY) domain.¹ While a function has not yet been assigned to TRIM5γ, it is known that expression of TRIM5γ variants from humans, rhesus monkeys, and African green monkeys enable resistance to infection by various retroviruses including HIV-1, ^{2, 3} albeit at differing efficiencies. Furthermore, the TRIM5γ isoform appears to serve as a scaffold for the assembly of endogenous BTBD1 and BTBD2 proteins and also exhibits autoubiquitination activity in a RING finger- and UbcH5B-dependent manner.⁴

Antigen Details

Protein

TRIM5γ

PubMed

TRIM5γ

References & Citations

1. Reymond, A. et al. (2001) EMBO J. 20:2140
2. Stremlau, M. et al. (2004) Nature 427:848
3. Hatziioannou, T. et al. (2004) Proc. Nat’l. Acad. Sci. USA 101:10774
4. Xu, L. et al. (2003) Exp. Cell Res. 288:84

Technical Protocols