Anti-Human TL-1A

Antibody Details

Product Details

Reactivity Species

Human

Host Species

Goat

Immunogen

Purified Recombinant Human TL-1A (> 98%)

Endotoxin Level

< 0.1 EU/µg as determined by the LAL method

Formulation

This antigen affinity purified polyclonal antibody has been 0.2 µm filtered and lyophilized from modified Dulbecco’s phosphate buffered saline (1X PBS) pH 7.2 – 7.3 containing 5.0% w/v trehalose with no calcium, magnesium, or preservatives present.

Storage and Handling

The lyophilized antigen affinity purified polyclonal antibody can be stored desiccated at -20°C to -70°C for twelve months from date of receipt. The reconstituted antibody can be stored for at least four weeks at 2-8°C. For long-term storage of the reconstituted antibody, aseptically aliquot into working volumes and store at -20°C to -70°C in a manual defrost freezer. Avoid Repeated Freeze Thaw Cycles. No detectable loss of activity was observed after six months.

Country of Origin

USA

Shipping

Next Day Ambient

RRID

AB_2831858

Applications and Recommended Usage?
Quality Tested by Leinco

Western Blot: Suitable for use at concentration of 0.1 µg/mL.

Other Applications Reported In Literature ?

Immunohistochemistry: Suitable for use at concentration of 3-15 µg/mL.

Each investigator should determine their own optimal working dilution for specific applications. See directions on lot specific datasheets, as information may periodically change.

Description

Specificity

Goat Anti-Human TNF-Ligand-Related Molecule 1-A (TL1-A) recognizes Human TL1-A. This antigen affinity purified polyclonal antibody was purified using a proprietary chromatographic technique that includes covalently immobilizing the antigen proteins or peptides to agarose based beads. This purification method enhances specificity, reduces nonspecific binding of extraneous IgG and provides you with the most reliable reagent available for your early discovery research.

Antigen Distribution

Human TL-1A is expressed predominantly in endothelial cells, and to a lesser extent in placenta, lung, kidney, skeletal muscle, pancreas, small intestine and colon.

Background

TNF-ligand-related molecule 1A (TL1A), is an endothelium-derived T cell costimulator and a ligand for tumor necrosis factor receptor superfamily members DR3 and decoy receptor 3.¹ TL1A binds to the death-domain receptor (DR)3 and provides costimulatory signals to activated lymphocytes. Through this interaction, TL1A induces secretion of IFN-γ. Blockade of the TL1A/DR3 pathway may offer therapeutic opportunities in Crohn's disease.²

Antigen Details

Protein

TL1-A

PubMed

TL1-A

NCBI Gene Bank ID

9966

References & Citations

1. Wei, P. et al. (2003) J Biol Chem. 278: 39251

2. Cominelli, F.et al. (2006) PNAS 103: 8441

Technical Protocols