Anti-Human SCF

Antibody Details

Product Details

Reactivity Species

Human

Host Species

Goat

Immunogen

Purified Recombinant Human SCF (> 98%)

Endotoxin Level

< 0.1 EU/µg as determined by the LAL method

Formulation

This antigen affinity purified polyclonal antibody has been 0.2 µm filtered and lyophilized from modified Dulbecco’s phosphate buffered saline (1X PBS) pH 7.2 – 7.3 containing 5.0% w/v trehalose with no calcium, magnesium, or preservatives present.

Storage and Handling

The lyophilized antigen affinity purified polyclonal antibody can be stored desiccated at -20°C to -70°C for twelve months from date of receipt. The reconstituted antibody can be stored for at least four weeks at 2-8°C. For long-term storage of the reconstituted antibody, aseptically aliquot into working volumes and store at -20°C to -70°C in a manual defrost freezer. Avoid Repeated Freeze Thaw Cycles. No detectable loss of activity was observed after six months.

Country of Origin

USA

Shipping

Next Day Ambient

RRID

AB_2831743

Applications and Recommended Usage
Quality Tested by Leinco

Western Blot: Suitable for use at concentration of 0.1 µg/mL.

Other Applications Reported In Literature

Neutralization:Measured by its ability to neutralize SCF/c‑kit Ligand-induced proliferation in the TF‑1 human erythroleukemic cell line [Kitamura, T. et al. (1989) J. Cell Physiol. 140:323]. The Neutralization Dose (ND₅₀) is typically 0.05-0.3 µg/mL in the presence of 10 ng/mL Recombinant Human SCF/c‑kit Ligand.

Each investigator should determine their own optimal working dilution for specific applications. See directions on lot specific datasheets, as information may periodically change.

Description

Specificity

Rabbit Anti-Human Stem Cell Factor (SCF) recognizes Human SCF. This antigen affinity purified polyclonal antibody was purified using a proprietary chromatographic technique that includes covalently immobilizing the antigen proteins or peptides to agarose based beads. This purification method enhances specificity, reduces nonspecific binding of extraneous IgG and provides you with the most reliable reagent available for your early discovery research.

Background

Stem cell factor (SCF), otherwise known as KIT ligand or Steel factor¹ is a paracrine regulator of germ cell development.² SCF has the ability to stimulate the growth of hematopoietic progenitor cells and to generate colony-forming cells (CFC) from highly enriched populations of hematopoietic cells.³ SCF is produced by the Sertoli cells and involved in the local regulation of spermatogenesis⁴ and liver regeneration.⁵ SCF in conjunction with interleukin-31 may play a significant role in airway remodeling by promoting the recruitment of bone marrow-derived fibroblast precursors into the lung with the capacity to promote lung myofibroblast differentiation.⁶

Antigen Details

Protein

SCF

PubMed

SCF

NCBI Gene Bank ID

4254

UniProt.org

More Information on Uniprot.org

References & Citations

1. Toppari, J. et al. (1999) Endocrin 140: 1492

2. Adamson, JW. et al. (1991) PNAS 88: 7420

3. Hervé, L. et al. (2005) J of Endocrin. 186: 131

4. Ramadori, G. et al. (2009) Lab Invest. 89(5):562-74.

6. Phan, SH. et al. (2009) AM J Pathol. 174: 390

Technical Protocols