Anti-Human TRANCE - Biotin

Antibody Details

Product Details

Reactivity Species

Human

Host Species

Goat

Immunogen

Purified Recombinant Human sRANKL (Accession # O14788)

Formulation

This biotinylated antigen affinity purified polyclonal antibody has been 0.2 µm filtered and lyophilized from modified Dulbecco’s phosphate buffered saline (1X PBS) pH 7.2 – 7.3 containing 50 µg of bovine serum albumin per µg of antibody with no calcium, magnesium, or preservatives present.

Storage and Handling

The lyophilized, biotinylated antigen affinity purified polyclonal antibody can be stored desiccated at -20°C to -70°C for up to twelve months from date of receipt. The reconstituted biotin conjugate can be stored for at least four weeks at 2-8°C. For long-term storage of the reconstituted conjugate, aseptically aliquot into working volumes and store at -20°C to -70°C in a manual defrost freezer. Avoid Repeated Freeze Thaw Cycles. No detectable loss of activity was observed after six months.

Country of Origin

USA

Shipping

Next Day Ambient

RRID

AB_2831730

Applications and Recommended Usage?
Quality Tested by Leinco

Western Blot: Suitable for use at concentration of 0.1 µg/mL.

Each investigator should determine their own optimal working dilution for specific applications. See directions on lot specific datasheets, as information may periodically change.

Description

Specificity

Goat Anti-Human Soluble Receptor Activator of NFkB Ligand (sRANKL) recognizes Human sRANKL. This antigen affinity purified polyclonal antibody was purified using a proprietary chromatographic technique that includes covalently immobilizing the antigen proteins or peptides to agarose based beads. This purification method enhances specificity, reduces nonspecific binding of extraneous IgG and provides you with the most reliable reagent available for your early discovery research.

Background

RANKL (Receptor Activator for Nuclear Factor κ B Ligand), also known as TNF-related activation-induced cytokine (TRANCE), osteoprotegerin ligand (OPGL), and ODF (osteoclast differentiation factor), is a member of the tumor necrosis factor family cytokine.¹ RANKL is essential for osteoclast formation, activation, and survival.² RANKL through interaction with its receptor RANK can modulate matrix degradation and inflammation.³ RANKL and RANK have essential roles in lymph node formation, establishment of the thymic microenvironment, and development of a lactating mammary gland during pregnancy. Consequently, novel drugs specifically targeting RANK, RANKL, and their signaling pathways in osteoclasts are expected to revolutionize the treatment of various ailments associated with bone loss, such as arthritis, periodontal disease, cancer metastases, and osteoporosis.⁴

Antigen Details

Protein

TRANCE

PubMed

TRANCE

NCBI Gene Bank ID

8600

UniProt.org

More Information on Uniprot.org

References & Citations

1. Takayanagi, H. et al. (2003) Arthritis Res Ther. 5: 20

2. Kostenuik, PJ. et al. (2005) Endocrinology 146: 3235

3. Holven, KB. et al. (2009) Stroke 40: 241

4. Penninger, JM. et al. (2008) Ann N Y Acad Sci. 1143: 123

Technical Protocols