Goat F(ab’)2 Anti-Rat IgG, Fcgamma Fragment Specific (Adsorbed Against: Hu., Bv., Hs.) - DyLight 550

Antibody Details

Product Details

Reactivity Species

Rat

Host Species

Goat

Immunogen

Purified Rat IgG, Fc (Gamma) Fragment (> 98%)

Product Concentration

1.5 mg/ml

Formulation

This secondary antibody is lyophilized from 0.01M phosphate buffered saline (PBS) pH 7.6 containing 0.25M NaCl, 15 mg/ml IgG free BSA and 0.05% sodium azide as a preservative.

Product Preparation

This F(ab')₂ secondary antibody was generated by optimal pepsin degestion of the antisera followed by antigen affinity chromatography to elimiate the presence of Fc fragment and whole molecules.

Storage and Handling

This lyophilized secondary antibody conjugate should be stored desiccated at 2-8°C. The reconstituted DyLight® 549 conjugate containing glycerol should be stored at -20°C in a manual defrost freezer.

Expiration Date

One year from date of reconstitution

Country of Origin

USA

Shipping

Next Day Ambient

Working Concentration

It is recommended that working dilutions be prepared fresh daily. FC: 1:100 - 1:800IF: 1:100 - 1:800Secondary antibody concentrations reconstituted with glycerol will be diluted by 50%. Therefore, the working dilution may be one half of what is recommended.

RRID

AB_2894234

Each investigator should determine their own optimal working dilution for specific applications. See directions on lot specific datasheets, as information may periodically change.

Description

Specificity

Goat F(ab')₂ Anti-Rat IgG, Fc (Gamma) Fragment recognizes Rat IgG, Fc (Gamma) Fragment. This secondary antibody was purified using antigen affinity chromatography and has been adsorbed against serum proteins and immunoglobulins to minimize species cross-reactivity.

Antigen Details

Protein

Fc (Gamma) Fragment

PubMed

Fc (Gamma) Fragment