Goat F(ab')2 Anti-Rabbit IgG (H&L) (Adsorbed Against: Hu., Bv.) - Purified

Antibody Details

Product Details

Reactivity Species

Rabbit

Host Species

Goat

Immunogen

Purified Rabbit IgG (H& L) (> 98%)

Product Concentration

0.5 mg/ml

Formulation

This purified antibody is formulated in 0.01 M phosphate buffered saline (150 mM NaCl) PBS pH 7.4, 1% BSA and 0.09% sodium azide as a preservative.

Product Preparation

This F(ab')₂ secondary antibody was generated by optimal pepsin degestion of the antisera followed by antigen affinity chromatography to elimiate the presence of Fc fragment and whole molecules.

Storage and Handling

This secondary antibody conjugate should be stored for up to 4 weeks at 2-8°C. However, for long-term storage, aliquot into working volumes without dilution and store at –20°C in a manual defrost freezer. Avoid Repeated Freeze Thaw Cycles.

Country of Origin

USA

Shipping

Next Day 2-8°C

Working Concentration

This secondary reagent has been optimally manufactured to detect primary rabbit antibodies. Leinco Technologies uses F(ab')2 fragments to eliminate Fc receptor binding during use in flow cytometry or immunohistochemistry.

RRID

AB_2894170

Each investigator should determine their own optimal working dilution for specific applications. See directions on lot specific datasheets, as information may periodically change.

Description

Specificity

Goat F(ab')₂ Anti-Rabbit IgG (H& L) recognizes Rabbit IgG (H& L). This secondary antibody was purified using antigen affinity chromatography and has been adsorbed against serum proteins and immunoglobulins to minimize species cross-reactivity.

Antigen Details

Protein

IgG (H& L)

PubMed

IgG (H& L)