Antibody Details
Product Details
Host Species
Armenian Hamster
Product Concentration
≥ 5.0 mg/ml
Endotoxin Level
< 0.5 EU/mg as determined by the LAL method
Purity
≥98% monomer by analytical SEC
> 95% by SDS Page
Formulation
This monoclonal antibody is aseptically packaged and formulated in 0.01 M phosphate buffered saline (150 mM NaCl) PBS pH 7.2 - 7.4 with no carrier protein, potassium, calcium or preservatives added.
Product Preparation
Functional grade preclinical antibodies are manufactured in an animal free facility using only In vitro protein free cell culture techniques and are purified by a multi-step process including the use of protein A or G to assure extremely low levels of endotoxins, leachable protein A or aggregates.
Pathogen Testing
To protect mouse colonies from infection by pathogens and to assure that experimental preclinical data is not affected by such pathogens, all of Leinco’s Purified Functional PLATINUM™ antibodies are tested and guaranteed to be negative for all pathogens in the IDEXX IMPACT I Mouse Profile.
Storage and Handling
Functional grade preclinical antibodies may be stored sterile as received at 2-8°C for up to one month. For longer term storage, aseptically aliquot in working volumes without diluting and store at -80°C. Avoid Repeated Freeze Thaw Cycles.
Country of Origin
USA
Shipping
Next Day 2-8°C
Working Concentration
This isotype control antibody should be used at the same concentration as the primary antibody.
RRID
Applications and Recommended Usage
Quality Tested by Leinco
FC This isotype control antibody should be used at the same concentration as the primary antibody.
Each investigator should determine their own optimal working dilution for specific applications. See directions on lot specific datasheets, as information may periodically change.
Description
Specificity
This Armenian Hamster IgG isotype control antibody has been tested against selected species' cells and tissues to assure minimal cross reactivity. Furthermore, this Low Endotoxin Functional Formulation, PLATINUM antibody is suitable for in vivo application and each lot is IMPACT I certified.
References & Citations
1. Schreiber, RD. et al. (2017) Cancer Immunol Res. 5(2):106-117. PubMed
2. Oldstone, MBA. et al. (2017) Proc Natl Acad Sci U S A. 114(14): 3708–3713. PubMed
2. Schreiber, RD. et al. (2015) PLoS One.10(5):e0128636. PubMed
Technical Protocols