Anti-Human p34cdc2 Serine/Threonine Kinase - DyLight 650

Antibody Details

Product Details

Reactivity Species

Human

Host Species

Mouse

Immunogen

Purified Recombinant Human p34^cdc2 Serine/Threonine Kinase (> 98%)

Product Concentration

0.2 mg/ml

Formulation

This DyLight® 650 conjugate is formulated in 0.01 M phosphate buffered saline (150 mM NaCl) PBS pH 7.4, 1% BSA and 0.09% sodium azide as a preservative.

Storage and Handling

This DyLight^® 650 conjugate is stable when stored at 2-8°C. Do not freeze.

Country of Origin

USA

Shipping

Next Day 2-8°C

Excitation Laser

Red Laser (652 nm)

RRID

AB_2831603

Each investigator should determine their own optimal working dilution for specific applications. See directions on lot specific datasheets, as information may periodically change.

Description

Specificity

Mouse Anti-Human p34^cdc2 Serine/Threonine Kinase (Clone 17) recognizes an epitope on Human p34^cdc2 Serine/Threonine Kinase. This monoclonal antibody was purified using multi-step affinity chromatography methods such as Protein A or G depending on the species and isotype.

Background

p34^cdc2 is thought to be activated by cyclin through dephosphorylation at tyrosine residues. Activated p34^cdc2 kinase is involved in a number of mitotic events such as nuclear envelope breakdown and chromosome condensation through phosphorylation of serine/threonine residues of substrates such as nuclear lamins, histones, pp60c-src, nucleolin and RNA polymerase II. It is then inactivated by a tyrosine kinase. Anti-p34^cdc2 can be used in western blotting, immunoprecipitation and immunohistochemistry.
The antibody precipitates an active kinase.

Antigen Details

Protein

p34cdc2 Serine/Threonine Kinase

PubMed

p34cdc2 Serine/Threonine Kinase

NCBI Gene Bank ID

983

UniProt.org

Information on Uniprot.org

Research Area

Apoptosis

.

Cell Biology

References & Citations

1. Moreno, S. et al. (1990) Cell 61:549
2. Ward, GE. et al. (1990) Cell 61:561
3. Langan, TA. et al. (1989) Mol. Cell. Biol. 9:3860

Technical Protocols