Anti-Mouse PlGF-2

Antibody Details

Product Details

Reactivity Species

Mouse

Host Species

Goat

Immunogen

Sf 21-derived mouse PlGF-2

Formulation

This antigen affinity purified polyclonal antibody has been 0.2 µm filtered and lyophilized from modified Dulbecco’s phosphate buffered saline (1X PBS) pH 7.2 – 7.3 containing 5.0% w/v trehalose with no calcium, magnesium, or preservatives present.

Storage and Handling

The lyophilized antigen affinity purified polyclonal antibody can be stored desiccated at -20°C to -70°C for twelve months from date of receipt. The reconstituted antibody can be stored for at least four weeks at 2-8°C. For long-term storage of the reconstituted antibody, aseptically aliquot into working volumes and store at -20°C to -70°C in a manual defrost freezer. Avoid Repeated Freeze Thaw Cycles. No detectable loss of activity was observed after six months.

Country of Origin

USA

Shipping

Next Day Ambient

RRID

AB_2831571

Applications and Recommended Usage?
Quality Tested by Leinco

Western Blotting: To detect Mouse PlGF-2 this polyclonal antibody can be used at a concentration of 0.1-0.2 µg/ml. This polyclonal antibody should be used in conjunction with compatible second-step reagents such as PN:G505 and a chromogenic substrate such as PN:T343. The detection limit for Mouse PlGF-2 is 5 ng/lane under non-reducing and 25 ng/lane under reducing conditions. The sensitivity of detection may increase up to 50 fold when a chemiluminescent substrate is used. A suitable Western blotting control is PN:P255.

Each investigator should determine their own optimal working dilution for specific applications. See directions on lot specific datasheets, as information may periodically change.

Description

Specificity

Goat Anti-Mouse Placenta Growth Factor 2 (PlGF-2) recognizes Mouse PlGF-2. This antigen affinity purified polyclonal antibody was purified using a proprietary chromatographic technique that includes covalently immobilizing the antigen proteins or peptides to agarose based beads. This purification method enhances specificity, reduces nonspecific binding of extraneous IgG and provides you with the most reliable reagent available for your early discovery research.

Background

Placenta growth factor 2 (PlGF-2) is an isoform of PlGF¹ and a member of the vascular endothelial growth factor (VEGF) family. PlGF-2 has heparin binding properties and Neuropilin-1 is a receptor for PlGF-2.

Antigen Details

Protein

PlGF-2

PubMed

PlGF-2

NCBI Gene Bank ID

18654

UniProt.org

More Information on Uniprot.org

References & Citations

1. Neufeld, G. et al. (1998) J Biol Chem. 273: 22272

Technical Protocols