Anti-PCNA - APC

Antibody Details

Product Details

Reactivity Species

Rat

Host Species

Mouse

Immunogen

Rat PCNA made in the protein A vector pR1T2T

Product Concentration

0.2 mg/ml

Formulation

This Allophycocyanin (APC) conjugate is formulated in 0.01 M phosphate buffered saline (150 mM NaCl) PBS pH 7.4, 1% BSA and 0.09% sodium azide as a preservative.

Storage and Handling

This APC conjugate is stable when stored at 2-8°C. Do not freeze.

Country of Origin

USA

Shipping

Next Day Ambient

Excitation Laser

Red Laser (650 nm)

RRID

AB_2831519

Applications and Recommended Usage?
Quality Tested by Leinco

FC The suggested concentration for this anti-PCNA (Clone PC-10) antibody for staining cells in flow cytometry is ≤ 1.0 μg per 10⁶ cells in a volume of 100 μl or 100μl of whole blood. Titration of the reagent is recommended for optimal performance for each application.

Other Applications Reported In Literature ?

ICFC

Each investigator should determine their own optimal working dilution for specific applications. See directions on lot specific datasheets, as information may periodically change.

Description

Specificity

Clone PC10 recognizes an epitope on mouse PCNA.

Antigen Distribution

PCNA is a ubiquitous nuclear protein present in all cell cycle stages, with the highest expression in the early S phase. Anti-PCNA staining is almost entirely confined to the nucleus and may show a diffuse or granular pattern or a mixture of both.

Background

PCNA antibody, clone PC10, recognizes proliferating cell nuclear antigen (PCNA), also known as cyclin, a 36 kDa nuclear protein belonging to the DNA sliding clamp family. PCNA is a ring-shaped homotrimer that encircles and slides along DNA^{1, 2}. PCNA is an auxiliary protein of DNA polymerase δ and ε and tethers the polymerase catalytic unit to the DNA template for efficient DNA synthesis^3-6. PCNA also contributes to chromatin remodeling, DNA repair, sister chromatid cohesion, and cell cycle control⁷. Expression levels of PCNA are associated with proliferation and transformation, and PCNA is commonly used as a marker for proliferating cells^{8, 9}. Antibodies to PCNA are detected in sera from patients with systemic lupus erythematosus (SLE)¹⁰. In addition, PCNA is a reliable diagnostic and prognostic biomarker for cancer, as tumor cells express high levels of PCNA¹¹.

Antigen Details

Protein

PCNA

Ligand/Receptor

Ubiquitination, Sumoylation

Function

Increases DNA polymerase δ processibility during elongation of the leading strand.

PubMed

PCNA

NCBI Gene Bank ID

5111

Research Area

Cell Biology

.

Immunology

.

Neuroscience

.

Neuroscience Cell Markers

.

Stem Cell

References & Citations

1. Krishna TS, et al. (1994) Cell. 79(7):1233-43

2. Schurtenberger P, et al. (1998) J Mol Biol. 275(1):123-32

3. Kelman, Z. (1997) Oncogene. 14, 629–640

4. Tan CK, et al. (1986) J Biol Chem. 261(26):12310-6

5. Bravo R, et al. (1987) Nature. 8; 326(6112):515-7

6. Prelich G, et al. (1987) Nature. 8; 326(6112):517-20

7. Maga G, Hubscher U. (2003) J Cell Sci. 116(Pt 15):3051-60

8. Bravo R, et al. (1982) Prog Clin Biol Res. 85 Pt A:235-48.

9. Celis JE, et al. (1984) Leuk Res. 8(2):143-57

10. Miyachi K, et al. (1978) J Immunol. 121(6):2228-34.

11. Naryzhny SN, Lee H. (2007) FEBS Lett.581(25):4917-20

Technical Protocols