Anti-Human M-CSF R - Biotin

Antibody Details

Product Details

Reactivity Species

Human

Host Species

Goat

Immunogen

Purified Recombinant Human M-CSF R (Accession # CAA27300)

Formulation

This biotinylated antigen affinity purified polyclonal antibody has been 0.2 µm filtered and lyophilized from modified Dulbecco’s phosphate buffered saline (1X PBS) pH 7.2 – 7.3 containing 50 µg of bovine serum albumin per µg of antibody with no calcium, magnesium, or preservatives present.

Storage and Handling

The lyophilized, biotinylated antigen affinity purified polyclonal antibody can be stored desiccated at -20°C to -70°C for up to twelve months from date of receipt. The reconstituted biotin conjugate can be stored for at least four weeks at 2-8°C. For long-term storage of the reconstituted conjugate, aseptically aliquot into working volumes and store at -20°C to -70°C in a manual defrost freezer. Avoid Repeated Freeze Thaw Cycles. No detectable loss of activity was observed after six months.

Country of Origin

USA

Shipping

Next Day Ambient

RRID

AB_2831300

Applications and Recommended Usage?
Quality Tested by Leinco

Flow Cytometry: It is recommended to use the indirect method for signal enhancement when enumerating cells expressing M-CSF R. A suggested method would be to stain cells expressing M-CSF R with 3 - 10 µg per 1 x 10⁶ cells in a total staining volume of 100 µl followed by PN:A104.
Western Blotting: To detect Human M-CSF R this biotin conjugate can be used at a concentration of 0.1 - 0.2 µg/ml. This biotin conjugate should be used in conjunction with compatible second-step reagents such as PN:A106 and a chromogenic substrate such as PN:T343. The detection limit for Human M-CSF R is 5 ng/lane under either reducing or non-reducing conditions. The sensitivity of detection may increase up to 50 fold when a chemiluminescent substrate is used. A suitable Western blotting control is PN:M1237.
ELISA Sandwich Assay: This antibody can be used as the detection antibody in a sandwich ELISA at a concentration of approximately 0.1-0.4 µg/ml.

Each investigator should determine their own optimal working dilution for specific applications. See directions on lot specific datasheets, as information may periodically change.

Description

Specificity

Goat Anti-Human Macrophage Colony Stimulating Factor Receptor (M-CSF R) recognizes Human M-CSF R. This antigen affinity purified polyclonal antibody was purified using a proprietary chromatographic technique that includes covalently immobilizing the antigen proteins or peptides to agarose based beads. This purification method enhances specificity, reduces nonspecific binding of extraneous IgG and provides you with the most reliable reagent available for your early discovery research.

Background

Macrophage colony-stimulating factor (M-CSF, also termed CSF-1) receptor is an integral membrane tyrosine kinase, expressed in monocytes (macrophages and their progenitors) and drives growth and development of this blood cell lineage.^{1, 2, 3} It also triggers growth and development of mononuclear phagocytes and placental trophoblasts.⁴ M-CSF receptor is present in osteoclasts both at mRNA and protein levels. When M-CSF ligand specifically binds to the M-CSF receptor on osteoclasts, it induces receptor dimerization, activation and autophosphorylation of cytoplasmic tyrosine residues⁵ M-CSF and its receptor present as candidate therapeutic targets in states of inflammatory bone erosion.⁶

Antigen Details

Protein

M-CSF R

PubMed

M-CSF R

NCBI Gene Bank ID

1436

UniProt.org

More Information on Uniprot.org

References & Citations

1. Stanley, ER. et al. (1978) Nature 274: 168
2. Byrne, PV. et al. (1981) J. Cell. Biol. 91: 848
3. Bourette, RP. et al. (2000) Growth Factors 17: 155
4. Stanley, ER. et al. (1996) Bone 18: 355
5. Novak, U. et al. (1996) Oncogene 13: 2607
6. Teitelbaum, SL. et al. (2005) J Clin Invest. 115(12):3418-27.

Technical Protocols