Comparison

Anti-Human DR5 (Drozitumab) - Fc Muted

Item no. LEIN-LT705-500ug
Manufacturer Leinco Technologies
Amount 500 ug
Category
Type Antibody Primary
Specific against other
ECLASS 10.1 32160702
ECLASS 11.0 32160702
UNSPSC 12352203
Available
info

Antibody Details

Product Details

Reactivity Species

Human


Host Species

Human


Expression Host

HEK-293


FC Effector Activity

Muted


Immunogen

DR5 scFv

Product Concentration

≥ 5.0 mg/ml


Endotoxin Level

≤ 1.0 EU/mg as determined by the LAL method


Purity

≥95% monomer by analytical SEC


Formulation

This biosimilar antibody is aseptically packaged and formulated in 0.01 M phosphate buffered saline (150 mM NaCl) PBS pH 7.2 - 7.4 with no carrier protein, potassium, calcium or preservatives added.

Product Preparation

Functional grade preclinical antibodies are manufactured in an animal free facility using only In vitro protein free cell culture techniques and are purified by a multi-step process including the use of protein A or G to assure extremely low levels of endotoxins, leachable protein A or aggregates.

Pathogen Testing

To protect mouse colonies from infection by pathogens and to assure that experimental preclinical data is not affected by such pathogens, all of Leinco’s recombinant biosimilar antibodies are tested and guaranteed to be negative for all pathogens in the IDEXX IMPACT I Mouse Profile.

Storage and Handling

Functional grade biosimilar antibodies may be stored sterile as received at 2-8°C for up to one month. For longer term storage, aseptically aliquot in working volumes without diluting and store at -80°C. Avoid Repeated Freeze Thaw Cycles.

Regulatory Status

Research Use Only (RUO). Non-Therapeutic.

Country of Origin

USA


Shipping

Next Day 2-8°C

RRID

AB_2893998

Applications and Recommended Usage
Quality Tested by Leinco

FC The suggested concentration for Drozitumab biosimilar antibody for staining cells in flow cytometry is ≤ 0.25 μg per 106 cells in a volume of 100 μl. Titration of the reagent is recommended for optimal performance for each application.

Other Applications Reported In Literature

FA
Agonist
WB
IP
IF
ELISA

Each investigator should determine their own optimal working dilution for specific applications. See directions on lot specific datasheets, as information may periodically change.

Description

Specificity

This non-therapeutic biosimilar antibody uses the same variable region sequence as the therapeutic antibody Drozitumab. Clone PRO95780 recognizes human death receptor 5 (DR5). This product is for research use only.

Antigen Distribution

DR5 is widely expressed in adult and fetal tissues, with notably high expression on tumor cells.

Background

Drozitumab is a monoclonal antibody that specifically binds to human DR5. DR5 is expressed in a variety of solid tumors and hematologic malignancies, which are characteristically resistant to apoptosis. Drozitumab is a proapoptotic receptor agonists (PARA) that induces apoptosis in a variety of human cancer cell lines and xenograft models, both alone and in tandem with other antineoplastic agents.1 Apoptosis of cancer cells triggered by the activation of DR5, occurs without affecting most normal cell types. Anti-Human DR5 (Drozitumab) utilizes the same variable regions from the therapeutic antibody Drozitumab making it ideal for research projects.

Antigen Details

Protein

DR5

PubMed

DR5

NCBI Gene Bank ID

8795

UniProt.org

Information on Uniprot.org

Research Area

Apoptosis

.

Cell Death

.

Immunology

.

Tumor Suppressors


References & Citations

1. Mendelson, D. et al. (2010) Clin Cancer Res. 16(4):1256-63.

Technical Protocols



Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.

All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.

Amount: 500 ug
Available: In stock
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