Comparison

Anti-Human CD52 (Alemtuzumab) - Fc Muted

Item no. LEIN-LT205-500ug
Manufacturer Leinco Technologies
Amount 500 ug
Quantity options 500 ug Custom
Category
Type Antibody Primary
Applications WB, FC, IHC, ELISA, FA
Clone Campath-1H
Specific against other
ECLASS 10.1 32160702
ECLASS 11.0 32160702
UNSPSC 12352203
Available
info

Antibody Details

Product Details

Reactivity Species

Human/Rhesus Monkey/Cynomolgus Monkey


Host Species

Human


Expression Host

HEK-293


FC Effector Activity

Muted


Immunogen

Human lymphocytes.

Product Concentration

≥ 5.0 mg/ml


Endotoxin Level

≤ 1.0 EU/mg as determined by the LAL method


Purity

> 95% by SDS-PAGE and HPLC


Formulation

This biosimilar antibody is aseptically packaged and formulated in 0.01 M phosphate buffered saline (150 mM NaCl) PBS pH 7.2 - 7.4 with no carrier protein, potassium, calcium or preservatives added.

Product Preparation

Functional grade preclinical antibodies are manufactured in an animal free facility using only In vitro protein free cell culture techniques and are purified by a multi-step process including the use of protein A or G to assure extremely low levels of endotoxins, leachable protein A or aggregates.

Pathogen Testing

To protect mouse colonies from infection by pathogens and to assure that experimental preclinical data is not affected by such pathogens, all of Leinco’s recombinant biosimilar antibodies are tested and guaranteed to be negative for all pathogens in the IDEXX IMPACT I Mouse Profile.

Storage and Handling

Functional grade biosimilar antibodies may be stored sterile as received at 2-8°C for up to one month. For longer term storage, aseptically aliquot in working volumes without diluting and store at -80°C. Avoid Repeated Freeze Thaw Cycles.

Regulatory Status

Research Use Only (RUO). Non-Therapeutic.

Country of Origin

USA


Shipping

Next Day 2-8°C

RRID

AB_2893943

Applications and Recommended Usage
Quality Tested by Leinco

FC The suggested concentration for Alemtuzumab biosimilar antibody for staining cells in flow cytometry is ≤ 0.25 μg per 106 cells in a volume of 100 μl. Titration of the reagent is recommended for optimal performance for each application.

Other Applications Reported In Literature

IHC FFPE (Formalin-fixed paraffin-embedded tissue)
IHC FF (Fresh Frozen)
FA
WB
ELISA

Additional Reported Applications For Relevant Conjugates

CyTOF®

Each investigator should determine their own optimal working dilution for specific applications. See directions on lot specific datasheets, as information may periodically change.

Description

Specificity

This non-therapeutic biosimilar antibody uses the same variable region sequence as the therapeutic antibody Alemtuzumab. Clone Campath-1H recognizes human CD52. This product is for research use only.

Antigen Distribution

CD52 is primarily expressed on the surface of mature lymphocytes. Additionally, CD52 is present on most lymphoid derived malignancies. However, variable expression on Myeloma cells should be noted.

Background

Clone Campath-1H is a monoclonal antibody that specifically binds to CD52, a protein present on the surface of mature lymphocytes. However, this protein is not present on the stem cells that generated these lymphocytes. Alemtuzumab is targets and destroys mature lymphocytes containing CD-52, and is used to treat chronic lymphocytic leukemia (CLL) and multiple sclerosis. Anti-Human CD52 (Alemtuzumab) utilizes the same variable regions from the therapeutic antibody Alemtuzumab making it ideal for research projects.

Antigen Details

Protein

CD52

PubMed

CD52

NCBI Gene Bank ID

1043

UniProt.org

Information on Uniprot.org

Technical Protocols



Manufacturer - Target
 CD52

Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.

All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.

Amount: 500 ug
Available: In stock
available

Delivery expected until 12/13/2024 

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