Anti-Mouse CXCL1 (Clone 124014)

Antibody Details

Product Details

Reactivity Species

Mouse

Host Species

Rat

Immunogen

E. coli-Derived Recombinant Mouse KC

Endotoxin Level

< 0.1 EU/µg as determined by the LAL method

Formulation

This monoclonal antibody has been 0.2 µm filtered and lyophilized from modified Dulbecco's phosphate buffered saline (1X PBS) pH 7.2 - 7.3 containing 5.0% w/v trehalose with no calcium, magnesium or preservatives present.

Storage and Handling

The lyophilized antibody can be stored desiccated at -20°C to -70°C for up to twelve months. The reconstituted antibody can be stored for at least four weeks at 2-8°C. For long-term storage of the reconstituted antibody, aseptically aliquot into working volumes and store at -20°C to -70°C in a manual defrost freezer. Avoid repeated freeze thaw cycles. No detectable loss of activity was observed after six months.

Country of Origin

USA

Shipping

Next Day Ambient

RRID

AB_2831089

Applications and Recommended Usage?
Quality Tested by Leinco

Western Blotting: To detect Mouse KC this monoclonal antibody can be used at a concentration of 1-2 µg/ml. This monoclonal antibody should be used in conjunction with compatible second-step reagents such as PN:R377 and a chromogenic substrate such as PN:T343. The detection limit for Mouse KC is 5 ng/lane under either reducing or non-reducing conditions. The sensitivity of detection may increase up to 50 fold when a chemiluminescent substrate is used.

Other Applications Reported In Literature ?

Neutralization: This antibody is useful for neutralization of Mouse KC bioactivity. The antibody dose required to neutralize 50% (ND₅₀) of the biological activity of Mouse KC (at 30 ng/ml) is 1-5 µg/ml.

Each investigator should determine their own optimal working dilution for specific applications. See directions on lot specific datasheets, as information may periodically change.

Description

Specificity

Rat Anti-Mouse CXCL1 (Clone 124014) recognizes an epitope on Mouse CXCL1. This monoclonal antibody was purified using multi-step affinity chromatography methods such as Protein A or G depending on the species and isotype.

Background

Chemokine (C-X-C motif) ligand 1 (CXCL1), also known as GRO/KC, is a small cytokine with mitogenic properties belonging to the CXC chemokine family that was previously called GRO1 oncogene, GROα, Neutrophil-activating protein 3 (NAP-3) and melanoma growth stimulating activity, alpha (MSGA-α).¹ CXCL1 is expressed by macrophages, neutrophils and epithelial cells.² CXCL1 plays a role in spinal cord development by inhibiting the migration of oligodendrocyte precursors and is involved in the processes of angiogenesis, inflammation, wound healing.^{3, 4}

Antigen Details

Protein

CXCL1

PubMed

CXCL1

References & Citations

1. Sager, R. et al. (1990) Proc. Natl. Acad. Sci. U.S.A. 87: 7732

2. Grotendorst, GR. et al. (1990) Mol. Cell. Biol. 10: 5596

3. Miller, RH. et al. (2002) Cell 110: 373

4. Richmond, A. et al. (2000) J. Invest. Dermatol. 115: 234

Technical Protocols