Anti-Human IgM u-Chain Specific - DyLight 650

Antibody Details

Product Details

Reactivity Species

Human

Host Species

Mouse

Immunogen

Purified Recombinant Human IgM µ-Chain Specific (> 98%)

Product Concentration

0.2 mg/ml

Formulation

This DyLight® 650 conjugate is formulated in 0.01 M phosphate buffered saline (150 mM NaCl) PBS pH 7.4, 1% BSA and 0.09% sodium azide as a preservative.

Storage and Handling

This DyLight^® 650 conjugate is stable when stored at 2-8°C. Do not freeze.

Cross Reactivity

Cross-reactivity by ELISA against human myeloma proteins:
Human IgG1: 100%
Human IgG2: 100%
Human IgG3: 100%
Human IgG4: 100%
Human IgM: < 0.2%
Human IgA: < 0.1%
Human IgE: < 0.1%

There is no detectable binding to bovine, goat, horse sheep IgG.

NOTE: This Anti-Human IgG, Fc Fragment Specific (Clone HB57) when conjugated to HRP is validated for use in Enzyme Immunoassay for the detection of Human IgG, µ-Chain Specific (Part No.: I-1201)

Country of Origin

USA

Shipping

Next Day 2-8°C

Excitation Laser

Red Laser (652 nm)

RRID

AB_2893840

Applications and Recommended Usage?
Quality Tested by Leinco

FC This antibody has been quality control tested by immunofluorescent staining with flow cytometric analysis. For immunofluorescent staining, the suggested use of this reagent is ≤0.25 µg per million cells in 100 µl volume.

Each investigator should determine their own optimal working dilution for specific applications. See directions on lot specific datasheets, as information may periodically change.

Description

Specificity

Mouse Anti-Human IgM µ-Chain Specific (Clone HB57) recognizes an epitope on Human IgM µ-Chain Specific. This monoclonal antibody was purified using multi-step affinity chromatography methods such as Protein A or G depending on the species and isotype.

Antigen Distribution

Surface IgM is expressed on B-lymphocytes.

Antigen Details

Protein

IgM µ-Chain Specific

PubMed

IgM µ-Chain Specific

References & Citations

1. Rudich, S. M. et al. (1988) J. Exp. Med. 168:247

Technical Protocols