Anti-Human IL-17E (Clone 182203)

Antibody Details

Product Details

Reactivity Species

Human

Host Species

Mouse

Immunogen

Purified Recombinant Human IL-17E (Accession # Q9H293)

Formulation

This monoclonal antibody has been 0.2 µm filtered and lyophilized from modified Dulbecco's phosphate buffered saline (1X PBS) pH 7.2 - 7.3 containing 5.0% w/v trehalose with no calcium, magnesium or preservatives present.

Storage and Handling

The lyophilized antibody can be stored desiccated at -20°C to -70°C for up to twelve months. The reconstituted antibody can be stored for at least four weeks at 2-8°C. For long-term storage of the reconstituted antibody, aseptically aliquot into working volumes and store at -20°C to -70°C in a manual defrost freezer. Avoid repeated freeze thaw cycles. No detectable loss of activity was observed after six months.

Country of Origin

USA

Shipping

Next Day Ambient

RRID

AB_2830959

Applications and Recommended Usage?
Quality Tested by Leinco

Flow Cytometry: It is recommended to use the indirect method for signal enhancement when enumerating cells expressing IL-17E. A suggested method would be to stain cells expressing IL-17E with 2.5 µg/10⁶ cells in a total staining volume of µl followed by PN:M1189.
Western Blotting: To detect Human IL-17E this monoclonal antibody can be used at a concentration of 1-2 µg/ml. This monoclonal antibody should be used in conjunction with compatible second-step reagents such as PN:M114 and a chromogenic substrate such as PN:T343. The detection limit for Human IL-17E is 5 ng/lane under either reducing or non-reducing conditions. The sensitivity of detection may increase up to 50 fold when a chemiluminescent substrate is used. A suitable Western blotting control is PN:I-199.

Other Applications Reported In Literature ?

CyTOF-ready:Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.

Each investigator should determine their own optimal working dilution for specific applications. See directions on lot specific datasheets, as information may periodically change.

Description

Specificity

Mouse Anti-Human IL-17E (Clone 182203) recognizes an epitope on Human IL-17E. This monoclonal antibody was purified using multi-step affinity chromatography methods such as Protein A or G depending on the species and isotype.

Background

IL-17E, also known as IL-25, is a novel member of the IL-17 family of cytokines¹ and a unique pleiotropic cytokine and may be an important mediator of inflammatory and immune responses.² IL-17E has a heterodimeric receptor. The receptor is composed of two subunits IL-17RA and IL-17RB. Although IL-17E only binds IL-17RB directly, both subunit receptors are essential for IL-17E functions. IL-17E induces activation of NF-κB and stimulates production of the proinflammatory chemokine IL-8. ¹ IL-17E may contribute to the induction and maintenance of eosinophilic inflammation in the airways by acting on lung fibroblasts and therefore has a role in asthma pathophysiology.³

Antigen Details

Protein

IL-17E

PubMed

IL-17E

NCBI Gene Bank ID

64806

UniProt.org

Information on Uniprot.org

Technical Protocols