Antibody Details
Product Details
Reactivity Species
Human
Host Species
Mouse
Immunogen
NS0-Derived Recombinant Human IGFBP-4 (Accession # AAA62670)
Endotoxin Level
< 0.1 EU/µg as determined by the LAL method
Formulation
This monoclonal antibody has been 0.2 µm filtered and lyophilized from modified Dulbecco's phosphate buffered saline (1X PBS) pH 7.2 - 7.3 containing 5.0% w/v trehalose with no calcium, magnesium or preservatives present.
Storage and Handling
The lyophilized antibody can be stored desiccated at -20°C to -70°C for up to twelve months. The reconstituted antibody can be stored for at least four weeks at 2-8°C. For long-term storage of the reconstituted antibody, aseptically aliquot into working volumes and store at -20°C to -70°C in a manual defrost freezer. Avoid repeated freeze thaw cycles. No detectable loss of activity was observed after six months.
Country of Origin
USA
Shipping
Next Day Ambient
RRID
Other Applications Reported In Literature ?
Neutralization:Measured by its ability to neutralize IGFBP‑4 inhibition of IGF‑II-dependent proliferation in the MCF‑7 human breast cancer cell line. Karey, K. P. et al. (1988) Cancer Research 48:4083. The Neutralization Dose (ND50) is typically 10‑40 µg/mL in the presence of 0.3 µg/mL Recombinant Human IGFBP‑4 and 14 ng/mL Recombinant Human IGF‑II.
Each investigator should determine their own optimal working dilution for specific applications. See directions on lot specific datasheets, as information may periodically change.
Description
Specificity
Mouse Anti-Human Insulin-Like Growth Factor Binding Protein 4 (IGFBP-4) (Clone 82334) recognizes an epitope on Human IGFBP-4. This monoclonal antibody was purified using multi-step affinity chromatography methods such as Protein A or G depending on the species and isotype.
Background
Insulin-like growth factor binding protein 4, also known as IGFBP4, is a member of the insulin-like growth factor binding protein (IGFBP) family that are cysteine-rich proteins that act as a carrier protein. The protein binds both insulin-like growth factors (IGFs) I and II and circulates in the plasma in both glycosylated and non-glycosylated forms. Binding of this protein prolongs the half-life of the IGFs and alters their interaction with cell surface receptors. IGFBP-4 is a unique protein and it consistently inhibits several cancer cells in vivo and in vitro. The protein itself does not prevent the formation of cancer.1 However it may reduce the growth of cancer and act as an apoptotic factor.
Antigen Details
Protein
IGFBP-4
PubMed
IGFBP-4
NCBI Gene Bank ID
3487
UniProt.org
Information on Uniprot.org
References & Citations
1. Winslet, MC. et al. (2007) Human Reproduction 5: 128
Technical Protocols