Anti-Mouse IFN-gamma (Clone XMG1.2) - PE

Antibody Details

Product Details

Reactivity Species

Mouse

Host Species

Rat

Immunogen

Purified Recombinant Mouse IFN-γ (> 98%)

Product Concentration

0.2 mg/ml

Formulation

This R-phycoerythrin (R-PE) conjugate is formulated in 0.01 M phosphate buffered saline (150 mM NaCl) PBS pH 7.4, 1% BSA and 0.09% sodium azide as a preservative.

Storage and Handling

This R-phycoerythrin (R-PE) conjugate is stable when stored at 2-8°C. Do not freeze.This R-PE conjugate is stable when stored at 2-8°C. Do not freeze.

Country of Origin

USA

Shipping

Next Day 2-8°C

Excitation Laser

Blue Laser (488 nm) and/or Green Laser (532 nm)/Yellow-Green Laser (561 nm)

RRID

AB_2830459

Other Applications Reported In Literature ?

ICFC The suggested concentration for clone XMG1.2 PE conjugated antibody for staining cells in Intracellular Immunofluorescent flow cytometry is ≤ .25 μg per 10⁶ cells in a volume of 100 μl or 100μl of whole blood. Titration of the reagent is recommended for optimal performance for each application.

Each investigator should determine their own optimal working dilution for specific applications. See directions on lot specific datasheets, as information may periodically change.

Description

Specificity

Rat Anti-Mouse Interferon Gamma (IFN-γ) (Clone XMG1.2) recognizes an epitope on Mouse IFN-γ. This monoclonal antibody was purified using multi-step affinity chromatography methods such as Protein A or G depending on the species and isotype.

Background

Interferon-gamma (IFN-γ) or type II interferon is a dimerized soluble cytokine that is the only member of the type II class of interferons.¹ It is a cytokine critical for innate and adaptive immunity against viral and intracellular bacterial infections and for tumor control. IFNG is produced predominantly by natural killer (NK) and natural killer T (NKT) cells as part of the innate immune response, and by CD4 and CD8 cytotoxic T lymphocyte (CTL) effector T cells once antigen-specific immunity develops.² IFN-γ has antiviral, immunoregulatory, and anti-tumour properties.³

Antigen Details

Protein

IFN-γ

PubMed

IFN-γ

NCBI Gene Bank ID

15978

UniProt.org

Information on Uniprot.org

Research Area

Cell Biology

.

Immunology

.

Neuroinflammation

.

Neuroscience

References & Citations

1. Goeddel DV et al. (1982) Nature 298: 859
2. Wilson CB et al. (2007) Adv. Immunol. 96: 41
3. Hume DA et al. (2004) J Leukoc Biol. 75: 163
4. Teijaro et al. (2020) bioRxiv 22: 24
5. Feldmann et al. (2021) Microorganisms 9(2): 279

Technical Protocols