Goat Anti-Human IgG (H&L) (Adsorbed Against: Ms., Bv., Hs.) - HRP

Antibody Details

Product Details

Reactivity Species

Human

Host Species

Goat

Immunogen

Purified Human IgG (H& L) (> 98%)

Product Concentration

0.8 mg/ml

Formulation

This secondary antibody is lyophilized from 0.01M phosphate buffered saline (PBS) pH 7.6 containing 0.25M NaCl, 15 mg/ml IgG free BSA. (Warning: Use of sodium azide as a preservative will inhibit the enzyme activity of horseradish peroxidase)

Storage and Handling

This lyophilized secondary antibody conjugate should be stored desiccated at 2-8°C. Caution: This horseradish peroxidase conjugate does not contain preservatives. To avoid microbial contamination, store the reconstituted conjugate containing glycerol at -20°C in a manual defrost freezer.

Expiration Date

One year from date of reconstitution

Country of Origin

USA

Shipping

Next Day USA Ambient $25

Working Concentration

It is recommended that working dilutions be prepared fresh daily. WB: 1:5, 000 - 1: 100, 000WB Chemiluminescent: 1:10, 000 - 1:200, 000ELISA: 1:5, 000 - 1: 100, 000IHC: 1:500 - 1:5, 000Secondary antibody concentrations reconstituted with glycerol will be diluted by 50%. Therefore, the working dilution may be one half of what is recommended.

RRID

AB_2892914

Each investigator should determine their own optimal working dilution for specific applications. See directions on lot specific datasheets, as information may periodically change.

Description

Specificity

Goat Whole Molecule Anti-Human IgG (H& L) recognizes Human IgG (H& L). This secondary antibody was purified using antigen affinity chromatography and has been adsorbed against serum proteins and immunoglobulins to minimize species cross-reactivity.

Antigen Details

Protein

IgG (H& L)

PubMed

IgG (H& L)